ArticleAnalytica chimica acta2019
Sorting by interfacial tension (SIFT): Label-free enzyme sorting using droplet microfluidics.
Article in Analytica chimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Optimization and Parallelization of Sorting by Interfacial Tension (SIFT) for High-Throughput Metabolic Cell Sorting.Micromachines · 2026Article
- Cell-in-Bead-in-Droplet Platform for pH-Based Microfluidic Screening of Ureolytic Bacteria.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Stepwise isolation of diverse metabolic cell populations using sorting by interfacial tension (SIFT).Lab on a chip · 2025Article
- Demand-driven active droplet generation and sorting based on positive pressure-controlled fluid wall.Analytical and bioanalytical chemistry · 2023Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform.Analytical chemistry · 2022Article
- Droplet Microfluidic Technology for the Early and Label-Free Isolation of Highly-Glycolytic, Activated T-Cells.Micromachines · 2022Article
- Recent Advances on Sorting Methods of High-Throughput Droplet-Based Microfluidics in Enzyme Directed Evolution.Frontiers in chemistry · 2021Review
- Method for Passive Droplet Sorting after Photo-Tagging.Micromachines · 2020Article
- High-throughput screening by droplet microfluidics: perspective into key challenges and future prospects.Lab on a chip · 2020Article
- Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Droplet microfluidics has the ability to greatly increase the throughput of screening and sorting of enzymes by carrying reagents in picoliter droplets flowing in inert oils. It was found with the use of a specific surfactant, the interfacial tension of droplets can be very sensitive to droplet pH. This enables the sorting of droplets of different pH when confined droplets encounter a microfabricated trench. The device can be extended to sort enzymes, as a large number of enzymatic reactions lead to the production of an acidic or basic product and a concurrent change in solution pH. The progress of an enzymatic reaction is tracked from the position of a flowing train of droplets. We demonstrate the sorting of esterase isoenzymes based on their enzymatic activity. This label-free technology, that we dub droplet sorting by interfacial tension (SIFT), requires no active components and would have applications for enzyme sorting in high-throughput applications that include enzyme screening and directed evolution of enzymes.
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