Evidence map›Paper›PMID 31626892›Full record

ArticleJournal of microbiological methods2019

Rapid viability polymerase chain reaction method for detection of Francisella tularensis.

Staci R Kane, Sanjiv R Shah, Teneile M Alfaro

Abstract read
In one paragraph

Article in Journal of microbiological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Unlocking the molecular realm: advanced approaches for identifying clinically and environmentally relevant bacteria.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023
    Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Staci R KaneLawrence Livermore National Laboratory, Livermore, CA, USA.
Sanjiv R ShahNational Homeland Security Research Center, Office of Research and Development, U.S. Environmental Protection Agency, Washington, DC, USA. Electronic address: shah.sanjiv@epa.gov.
Teneile M AlfaroLawrence Livermore National Laboratory, Livermore, CA, USA.

Funding

Intramural EPA EPA999999
6 · The paper itself

Abstract

Francisella tularensis, which causes potentially fatal tularemia, has been considered an attractive agent of bioterrorism and biological warfare due to its low infectious dose, reported environmental persistence, and ability to be transmitted to humans via multiple exposure routes. Due to slow growth on even selective culture media, detection of viable F. tularensis from environmental and drinking water samples, usually takes >3 days. Therefore, a rapid viability polymerase chain reaction (RV-PCR) method was developed to detect and identify viable F. tularensis cells in environmental samples. The method uses a change in PCR response during high throughput (48-well) sample incubation in Brain Heart Infusion/Vitox/Fildes/Histidine growth medium to detect viable F. tularensis presence, which is at least two times faster than the current plate culture-based method. Using the method, 10

Indexed as

Biological MonitoringBioterrorismDNA, BacterialFrancisella tularensisHumansPolymerase Chain ReactionTularemiaWater MicrobiologyDNA, BacterialBioterrorismContaminationDetectionFrancisella tularensisRV-PCRTularemia

Identifiers

PMID31626892
PMCPMC6951438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.