ArticleCell2019
Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.
Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
152 citing papers in PubMed, 256 citations in OpenAlex.
- Article
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Lineage tracing in gastruloids to understand determinants of early fate decisions.Current opinion in genetics & development · 2026Review
- Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.FEBS letters · 2026Article
- Secreted protein combination GAPDH/S100A8/S100A9 from human expanded potential stem cells counteracts mesenchymal stromal cell senescence.Stem cell research & therapy · 2026Article
- Article
- The vertebrate yolk sac: evolutionary origins and current advances in in vitro models.Development (Cambridge, England) · 2026Review
- Synthetic embryos: current progress, application and challenges.Reproductive biology and endocrinology : RB&E · 2026Review
- From Preservation to Creation: The Expanding Frontier of Fertility Preservation - Proceedings of the 2Facts, views & vision in ObGyn · 2026Article
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- New models of implantation: towards a whole better than the sum of parts.Human reproduction (Oxford, England) · 2026Review
- Depletion of Sorcs3 Activates Totipotency in Mouse Embryonic Stem Cells by Modulating Key Signaling Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Efficient stem cell-derived mouse embryo models for environmental studies.Developmental cell · 2026Article
- Use of miR-200-Mediated Reprogramming and Mechanical Cues to Generate Human Blastocyst Models.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Modelling mammalian implantationAnimal reproduction · 2026Review
- Impact of TGF-β and ERK1/2 inhibition on preimplantation embryo development and quality.Iranian journal of basic medical sciences · 2026Article
- Generation of Porcine Blastoids from Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026Review
- Developing stem cell-based bovine embryos for in vitro breeding.The Journal of reproduction and development · 2026Review
- BMP4 and FGF2 promote heterogeneous priming including extraembryonic lineages in porcine expanded potential stem cells.Frontiers in cell and developmental biology · 2026Article
92 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 3 countries.
Funding
Abstract
A single mouse blastomere from an embryo until the 8-cell stage can generate an entire blastocyst. Whether laboratory-cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to creating viable synthetic embryos by using cultured cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.