Evidence map›Paper›PMID 31625103›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.

Lawrence A Stern, Patrick S Lown, Benjamin J Hackel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lawrence A SternDepartment of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Patrick S LownDepartment of Chemical Engineering and Materials Science, University of Minnesota-Twin Cities, Minneapolis, MN, USA.
Benjamin J HackelDepartment of Chemical Engineering and Materials Science, University of Minnesota-Twin Cities, Minneapolis, MN, USA. hackel@umn.edu.

Funding

Engineering Gp2 as a small ligand scaffoldR01EB023339 · NIBIB · UNIVERSITY OF MINNESOTA · PI HACKEL, BENJAMIN · 2017 to 2020
$1.3M
NIBIB NIH HHS R01 EB023339
6 · The paper itself

Abstract

High-throughput ligand discovery and evolution-via genotype-phenotype linkage strategies-empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

Indexed as

Peptide LibraryProtein EngineeringSaccharomyces cerevisiaeAnimalsHumansLigandsLigandsPeptide LibraryAvidityCell panningDepletionLigandProtein engineeringSpecificityYeast surface display

Identifiers

PMID31625103
PMCPMC6996137

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.