Evidence map›Paper›PMID 31624872›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2019

Mutations in sorghum SBEIIb and SSIIa affect alkali spreading value, starch composition, thermal properties and flour viscosity.

Stefanie Griebel, Richard P Westerman, Adedayo Adeyanju, Charles Addo-Quaye, Bruce A Craig, Clifford F Weil, Suzanne M Cunningham, Bhavesh Patel, Osvaldo H Campanella, Mitchell R Tuinstra

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Stefanie GriebelDepartment of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
Richard P WestermanCollege of Agriculture Administration, Purdue University, West Lafayette, IN, 47907, USA.
Adedayo AdeyanjuDepartment of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
Charles Addo-QuayeDivision Natural Sciences and Mathematics, Lewis-Clark State College, Lewiston, ID, 83501, USA.
Bruce A CraigDepartment of Statistics, Purdue University, West Lafayette, IN, 47907, USA.
Clifford F WeilDepartment of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
Suzanne M CunninghamDepartment of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA.
Bhavesh PatelWhistler Carbohydrate Research Center, Purdue University, West Lafayette, IN, 47907, USA.
Osvaldo H CampanellaWhistler Carbohydrate Research Center, Purdue University, West Lafayette, IN, 47907, USA.
Mitchell R TuinstraDepartment of Agronomy, Purdue University, Lilly Hall of Life Sciences, 915 W State Street, West Lafayette, IN, 47907, USA. drmitch@purdue.edu.

Funding

United States Agency for International Development Cooperative Agreement No. AID-OAA-A-13-00047
6 · The paper itself

Abstract

key messageSeven novel alleles of SBEIIb and one allele of SSIIa co-segregated with the ASV phenotype and contributed to distinct starch quality traits important for food-processing applications. Sorghum is an important food crop for millions of people in Africa and Asia. Whole-genome re-sequencing of sorghum EMS mutants exhibiting an alkali spreading value (ASV) phenotype revealed candidate SNPs in Sobic.004G163700 and Sobic.010G093400. Comparative genomics identified Sobic.010G093400 as a starch synthase IIa and Sobic.004G163700 as a starch branching enzyme IIb. Segregation analyses showed that mutations in Sobic.010G093400 or Sobic.004G163700 co-segregated with the ASV phenotype. Mutants in SSIIa exhibited no change in amylose content but expressed lower final viscosity and lower starch gelatinization temperature (GT) than starches from non-mutant plants. The sbeIIb mutants exhibited significantly higher amylose levels and starch GT and lower viscosity compared to non-mutant starches and ssIIa mutants. Mutations in SBEIIb had a dosage-dependent effect on amylose content. Double mutants of sbeIIb and ssIIa resembled their sbeIIb parent in amylose content, starch thermal properties and viscosity profiles. These variants will provide opportunities to produce sorghum varieties with modified starch end-use qualities important for the beer brewing and baking industries and specialty foods for humans with diabetes.

Indexed as

1,4-alpha-Glucan Branching EnzymeAlkaliesAllelesAmyloseDNA Mutational AnalysisFlourGene DosageMutationPhenotypePlant ProteinsPolymorphism, Single NucleotideSequence AlignmentSorghumStarchStarch SynthaseViscosity1,4-alpha-Glucan Branching EnzymeAlkaliesAmylosePlant ProteinsStarchstarch-branching enzyme IIbStarch Synthasestarch synthase II

Identifiers

PMID31624872
PMCPMC6820604

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.