ArticlePlant methods2019
Spatially resolved transcriptomics reveals plant host responses to pathogens.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Enrichment is not necessarily recruitment in root microbiome assembly.The ISME journal · 2026Review
- RNA tomography reveals spatial gene expression maps of Arabidopsis thaliana roots infected with Heterodera schachtii.The New phytologist · 2026Article
- From pixels to cell types: a comprehensive review of computational methods for spatial transcriptomics deconvolution.Genomics & informatics · 2025Review
- Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses.The New phytologist · 2025Article
- Aphid effectors suppress plant immunity via recruiting defense proteins to processing bodies.Science advances · 2025Article
- Unravelling tumour spatiotemporal heterogeneity using spatial multimodal data.Clinical and translational medicine · 2025Review
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Spatiotemporal transcriptomics reveals key gene regulation for grain yield and quality in wheat.Genome biology · 2025Article
- Prunus dulcis response to novel defense elicitor peptides and control of Xylella fastidiosa infections.Plant cell reports · 2024Article
- Advances in Single-Cell Transcriptome Sequencing and Spatial Transcriptome Sequencing in Plants.Plants (Basel, Switzerland) · 2024Review
- STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization.Nucleic acids research · 2024Article
- Updates on the Immune Cell Basis of Hepatic Ischemia-Reperfusion Injury.Molecules and cells · 2023Review
- High expression of the MADS-box gene VRT2 increases the number of rudimentary basal spikelets in wheat.Plant physiology · 2022Article
- An in situ sequencing approach maps PLASTOCHRON1 at the boundary between indeterminate and determinate cells.Plant physiology · 2022Article
- Different threats, same response.Nature plants · 2021Article
- A low-cost pipeline for soil microbiome profiling.MicrobiologyOpen · 2020Article
- Precision transcriptomics of viral foci reveals the spatial regulation of immune-signaling genes and identifies RBOHD as an important player in the incompatible interaction between potato virus Y and potato.The Plant journal : for cell and molecular biology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundThorough understanding of complex model systems requires the characterisation of processes in different cell types of an organism. This can be achieved with high-throughput spatial transcriptomics at a large scale. However, for plant model systems this is still challenging as suitable transcriptomics methods are sparsely available. Here we present GaST-seq (
resultsWe compare the GaST-seq method with widely used library preparation methods (Illumina TruSeq). In spatial experiments we show that the GaST-seq method is sensitive enough to identify expression differences across a plant organ. We further assess the spatial transcriptome response of
conclusionWe show that our method can be used to identify known, rapidly flagellin-22 elicited genes, plant immune response pathways to bacterial attack and spatial expression patterns of genes associated with these pathways.
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