ArticleScientific reports2019
The impact of decellularization methods on extracellular matrix derived hydrogels.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 138 papers, 1 of them a synthesis that pooled it.
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Who cites it
138 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Treating chronic diarrhea: A systematic review on Immunoproliferative Small Intestinal Disease (IPSID).PloS one · 2021Pooled it
- Toward More Translational Tumor Models: Breast dECM-Based 3D Systems Capture Native Microenvironmental Cues.Bioengineering (Basel, Switzerland) · 2026Article
- Respiratory Organ-on-a-Chip for Disease Modeling: From Architecture to Functional Integration.Advanced healthcare materials · 2026Review
- Assembly of bioinspired multifunctional microspheres for enhanced alveolar bone regeneration.Bioactive materials · 2026Article
- Electrospun polyurethane-eggshell membrane hybrid nanofibrous scaffolds for enhanced fibroblast adhesion and growth in skin tissue engineering.Scientific reports · 2026Article
- Photocrosslinkable lung dECM hydrogels promote stiffness-dependent lung cancer growth and chemoresistance.Materials today. Bio · 2026Article
- Methods and Technical Issues for Optimizing the Production of Hydrogels Containing Decellularized Wharton's Jelly.ACS biomaterials science & engineering · 2026Article
- Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source.Regenerative therapy · 2026Review
- The mechanical strain as a cross-linking agent for skeletal muscle ECM-derived hydrogels.Materials today. Bio · 2026Article
- Immunomodulatory role of decellularized extracellular matrix in skin wound healing.Materials today. Bio · 2026Review
- Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells forIranian journal of basic medical sciences · 2026Article
- Physical decellularization of fish skin utilizing electrical fields.Regenerative biomaterials · 2026Article
- Extracellular-Matrix-Based Materials from Decellularized Tissue: Opportunities, Challenges, and Future Directions in Regenerative Medicine.Advanced healthcare materials · 2026Review
- Bone-Derived dECM Hydrogels Support Tunable Microenvironments for In Vitro Osteogenic Differentiation.Advanced healthcare materials · 2026Article
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- LR-PRP-saturated bioactive bovine-derived decellularized pericardium accelerates osteogenesis in a rabbit bone defect model.Journal of materials science. Materials in medicine · 2025Article
- Technique for decellularization and characterization of rat esophageal extracellular matrix for potential application as a 3D scaffold.Bioengineered · 2025Article
- Article
- Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture.Scientific reports · 2025Article
- Growth Factor-Free Engineered Biphasic Scaffold for Enhanced Bone Regeneration.Annals of biomedical engineering · 2025Article
78 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue-derived decellularized biomaterials are ideal for tissue engineering applications as they mimic the biochemical composition of the native tissue. These materials can be used as hydrogels for cell encapsulation and delivery. The decellularization process can alter the composition of the extracellular matrix (ECM) and thus influence the hydrogels characteristics. The aim of this study was to examine the impact of decellularization protocols in ECM-derived hydrogels obtained from porcine corneas. Porcine corneas were isolated and decellularized with SDS, Triton X-100 or by freeze-thaw cycles. All decellularization methods decreased DNA significantly when measured by PicoGreen and visually assessed by the absence of cell nuclei. Collagen and other ECM components were highly retained, as quantified by hydroxyproline content and sGAG, by histological analysis and by SDS-PAGE. Hydrogels obtained by freeze-thaw decellularization were the most transparent. The method of decellularization impacted gelation kinetics assessed by turbidimetric analysis. All hydrogels showed a fibrillary and porous structure determined by cryoSEM. Human corneal stromal cells were embedded in the hydrogels to assess cytotoxicity. SDS decellularization rendered cytotoxic hydrogels, while the other decellularization methods produced highly cytocompatible hydrogels. Freeze-thaw decellularization produced hydrogels with the overall best properties.
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