ArticleScientific reports2019
Microtubule disruption changes endothelial cell mechanics and adhesion.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Anti-endothelial cell antibodies in pathogenesis of vasculitis.Frontiers in immunology · 2025Review
- Quadrant darkfield for label-free imaging of intracellular puncta.Journal of biomedical optics · 2024Article
- Quadrant darkfield (QDF) for label-free imaging of intracellular puncta.bioRxiv : the preprint server for biology · 2024Article
- Schizophrenia endothelial cells exhibit higher permeability and altered angiogenesis patterns in patient-derived organoids.Translational psychiatry · 2024Article
- Immunofluorescence study of cytoskeleton in endothelial cells induced with malaria sera.Malaria journal · 2024Article
- Recombinant human secretory leukocyte protease inhibitor (rhSLPI) coated titanium enhanced human osteoblast adhesion and differentiation.Scientific reports · 2023Article
- Measuring the linear viscoelastic regime of MCF-7 cells with a monolayer rheometer in the presence of microtubule-active anti-cancer drugs at high concentrations.Interface focus · 2022Article
- Measuring biological materials mechanics with atomic force microscopy - Determination of viscoelastic cell properties from stress relaxation experiments.Microscopy research and technique · 2022Article
- Early Pharmacological Profiling of Antiproliferative Compounds by Live Cell Imaging.Molecules (Basel, Switzerland) · 2022Article
- MPPInternational journal of molecular sciences · 2022Article
- Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics.iScience · 2022Article
- Tracking of Endothelial Cell Migration and Stiffness Measurements Reveal the Role of Cytoskeletal Dynamics.International journal of molecular sciences · 2022Review
- Article
- Colchicine Impacts Leukocyte Trafficking in Atherosclerosis and Reduces Vascular Inflammation.Frontiers in immunology · 2022Article
- Finite element analysis of the influence of cyclic strain on cells anchored to substrates with varying properties.Medical & biological engineering & computing · 2022Article
- Review
- Review
- Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells.Biochimica et biophysica acta. General subjects · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interest in studying the mechanical and adhesive properties of cells has increased in recent years. The cytoskeleton is known to play a key role in cell mechanics. However, the role of the microtubules in shaping cell mechanics is not yet well understood. We have employed Atomic Force Microscopy (AFM) together with confocal fluorescence microscopy to determine the role of microtubules in cytomechanics of Human Umbilical Vein Endothelial Cells (HUVECs). Additionally, the time variation of the adhesion between tip and cell surface was studied. The disruption of microtubules by exposing the cells to two colchicine concentrations was monitored as a function of time. Already, after 30 min of incubation the cells stiffened, their relaxation times increased (lower fluidity) and the adhesion between tip and cell decreased. This was accompanied by cytoskeletal rearrangements, a reduction in cell area and changes in cell shape. Over the whole experimental time, different behavior for the two used concentrations was found while for the control the values remained stable. This study underlines the role of microtubules in shaping endothelial cell mechanics.
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