ArticleBiophysical journal2019
Extent of Cell Confinement in Microtracks Affects Speed and Results in Differential Matrix Strains.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Mechanometabolism of cell adhesion: Vinculin regulates bioenergetics via RhoA-ROCK.The Journal of cell biology · 2026Article
- 3D bioprinting of cell-laden constructs: technologies, bioink design, and biomedical applications.Biomedical materials (Bristol, England) · 2025Review
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Engineering approaches for understanding mechanical memory in cancer metastasis.APL bioengineering · 2024Review
- Confinement primes cells for faster migration by polarizing active mitochondria.Nanoscale advances · 2023Article
- Cell jamming in a collagen-based interface assay is tuned by collagen density and proteolysis.Journal of cell science · 2023Article
- Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development.Gels (Basel, Switzerland) · 2023Article
- Mitochondrial transfer from cancer-associated fibroblasts increases migration in aggressive breast cancer.Journal of cell science · 2023Article
- Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA.Cell reports · 2023Article
- Self-assembly of mesoscale collagen architectures and applications in 3D cell migration.Acta biomaterialia · 2023Article
- Article
- AGE-breaker ALT711 reverses glycation-mediated cancer cell migration.Soft matter · 2022Article
- Link between glucose metabolism and epithelial-to-mesenchymal transition drives triple-negative breast cancer migratory heterogeneity.iScience · 2022Article
- Regulation of Tumor Invasion by the Physical Microenvironment: Lessons from Breast and Brain Cancer.Annual review of biomedical engineering · 2022Review
- Melanoma cells adopt features of both mesenchymal and amoeboid migration within confining channels.Scientific reports · 2021Article
- Cancer cell metabolic plasticity in migration and metastasis.Clinical & experimental metastasis · 2021Review
- Transduction of cell and matrix geometric cues by the actin cytoskeleton.Current opinion in cell biology · 2021Review
- Recent advances in understanding the role of metabolic heterogeneities in cell migration.Faculty reviews · 2021Review
- The Cancer Microenvironment: Mechanical Challenges of the Metastatic Cascade.Frontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
During metastasis, cancer cells navigate through a spatially heterogeneous extracellular matrix (ECM). Physical properties of ECM, including the degree of confinement, influence cell migration behavior. Here, utilizing in vitro three-dimensional collagen microtracks, we demonstrate that cell-ECM interactions, specifically the degree of spatial confinement, regulate migratory behavior. We found that cells migrate faster when they are fully confined, contacting all four walls (top, bottom, and two sides) of a collagen microtrack, compared with cells that are partially confined, contacting less than four walls. When fully confined, cells exhibit fewer but larger vinculin-containing adhesions and create greater strains in the surrounding matrix directed toward the cell body. In contrast, partially confined cells develop a more elongated morphology with smaller but significantly more vinculin-containing adhesions and displace the surrounding matrix less than fully confined cells. The resulting effect of increasing cell contractility via Rho activation is dependent on the number of walls with which the cell is in contact. Although matrix strains increase in both fully and partially confined cells, cells that are partially confined increase speed, whereas those in full confinement decrease speed. Together, these results suggest that the degree of cell-ECM contact during confined migration is a key determinant of speed, morphology, and cell-generated substrate strains during motility, and these factors may work in tandem to facilitate metastatic cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.