Evidence map›Paper›PMID 31623386›Full record

ArticleInternational journal of molecular sciences2019

Pituitary Hormones (FSH, LH, PRL, and GH) Differentially Regulate AQP5 Expression in Porcine Ovarian Follicular Cells.

Mariusz T Skowronski, Patrycja Mlotkowska, Damian Tanski, Ewa Lepiarczyk, Bartosz Kempisty, Lukasz Jaskiewicz, Chandra S Pareek, Agnieszka Skowronska

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Aquaporin-5 Dynamic Regulation.International journal of molecular sciences · 2023
    Review
  3. Article
  4. Changes inInternational journal of molecular sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mariusz T SkowronskiVeterinary Center, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland. skowron@umk.pl.ORCID 0000-0003-2775-9642
Patrycja MlotkowskaDepartment of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland. patrycja.mlotkowska@wp.pl.
Damian TanskiDepartment of Animal Anatomy and Physiology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland. damian.tanski@uwm.edu.pl.ORCID 0000-0001-5099-323X
Ewa LepiarczykDepartment of Human Physiology, School of Medicine, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland. ewa.lepiarczyk@uwm.edu.pl.
Bartosz KempistyDepartment of Histology and Embryology; Poznan University of Medical Sciences, 61-701 Poznan, Poland. bkempisty@ump.edu.pl.
Lukasz JaskiewiczDepartment of Human Physiology, School of Medicine, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland. lukasz.jaskiewicz@uwm.edu.pl.ORCID 0000-0002-0035-4314
Chandra S PareekVeterinary Center, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland. chandra.s.pareek@gmail.com.
Agnieszka SkowronskaDepartment of Human Physiology, School of Medicine, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland. agnieszka.skowronska@uwm.edu.pl.ORCID 0000-0002-2274-8867

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to examine the effect of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and growth hormone (GH) on Aquaporin 5 (AQP5) expression in granulosa (Gc) and theca cells (Tc) from medium (MF) and large (LF) ovarian follicles of pigs. The results showed that GH significantly decreased the expression of AQP5 in Gc from MF in relation to the control. In the Gc of large follicles, PRL stimulated the expression of AQP5. However, the increased expression of AQP5 in the Tc of LF was indicated by GH and PRL in relation to the control. A significantly higher expression of the AQP5 protein in the Gc from MF and LF was indicated by FSH and PRL. In co-cultures, an increased expression of AQP5 was observed in the Gc from LF incubated with LH, PRL, and GH. A significantly increased expression of AQP5 was also observed in co-cultures of Tc from all type of follicles incubated with LH, whereas PRL stimulated the expression of AQP5 in Tc from MF. Moreover, AQP5 protein expression increased in the co-culture isolated from MF and LF after treatment with FSH, LH, PRL, and GH. AQP5 immunoreactivity was observed in the cytoplasm, mainly in the perinuclear region and endosomes, as well as in the cell membranes of Gc and Tc from the LF and MF.

Indexed as

Gene Expression Regulation, PlantAnimalsAquaporin 5BiomarkersCoculture TechniquesFemaleFollicle Stimulating HormoneGranulosa CellsGrowth HormoneLuteinizing HormoneOvarian FolliclePituitary HormonesProlactinSwineTheca CellsAquaporin 5BiomarkersFollicle Stimulating HormoneGrowth HormoneLuteinizing HormonePituitary HormonesProlactinAquaporin 5gene and protein expressionin vitro studyovarian cellspig

Identifiers

PMID31623386
PMCPMC6801619

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.