ArticlePloS one2019
First identification of the benzimidazole resistance-associated F200Y SNP in the beta-tubulin gene in Ascaris lumbricoides.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- A historical and systematic overview ofParasitology · 2021Pooled it
- Thereaputic efficacy of single dose Albendazole against Ascaris lumbricoides, prevalence of re-infection, and its associated factors among school children at Northwest Ethiopia: an open-label trial study.BMC infectious diseases · 2026Trial
- Genetic diversity, population structure, and benzimidazole resistance profiling of Ascaris lumbricoides in northwestern Thailand.Parasitology research · 2026Article
- The impact of the termination of Lymphatic Filariasis mass drug administration on Soil-transmitted Helminth prevalence in school children in Malawi.PLoS neglected tropical diseases · 2026Article
- Molecular epidemiology of Ascaris lumbricoides following multiple rounds of community-wide treatment.Nature communications · 2025Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- The impact of an integrated intervention program combining drug therapy with water, sanitation, and hygiene (WASH) education on reinfection with intestinal parasitic infections among the Karen hill tribe in northern Thailand.Parasites & vectors · 2024Article
- Review
- New Insights on Tools for Detecting β-Tubulin Polymorphisms inAnimals : an open access journal from MDPI · 2024Article
- Worldwide absence of canonical benzimidazole resistance-associated mutations within β-tubulin genes from Ascaris.Parasites & vectors · 2024Article
- Reduced efficacy of single-dose albendazole against Ascaris lumbricoides, and Trichuris trichiura, and high reinfection rate after cure among school children in southern Ethiopia: a prospective cohort study.Infectious diseases of poverty · 2024Article
- Anthelmintic resistance in soil-transmitted helminths: One-Health considerations.Parasitology research · 2023Review
- In silico design of a polypeptide as a vaccine candidate against ascariasis.Scientific reports · 2023Article
- Transcriptome profiling of male and female Ascaris lumbricoides reproductive tissues.Parasites & vectors · 2022Article
- Sensitivity ofJournal of pesticide science · 2022Article
- Identification of key interactions of benzimidazole resistance-associated amino acid mutations in Ascaris β-tubulins by molecular docking simulations.Scientific reports · 2022Article
- Interactions of Caenorhabditis elegans β-tubulins with the microtubule inhibitor and anthelmintic drug albendazole.Genetics · 2022Article
- Prevalence of major nematodes and human factors that affect infection in the zebra dove in a closed cage system.Veterinary world · 2022Article
- A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematodeFrontiers in veterinary science · 2022Article
- Exploring the β-tubulin gene family in a benzimidazole-resistant Parascaris univalens population.International journal for parasitology. Drugs and drug resistance · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main control strategy for Ascaris lumbricoides is mass drug administration (especially with benzimidazoles), which can select strains of parasites resistant to treatment. Mutations in the beta-tubulin isotype-1 gene at codons 167, 198 and 200 have been linked to benzimidazole resistance in several nematodes. The mutation in codon 200 is the most frequent in different species of parasites, as previously observed in Necator americanus and Trichuris trichiura; however, this mutation has never been found in populations of A. lumbricoides. This study aimed to screen for single nucleotide polymorphisms (SNPs) in the beta-tubulin isotype-1 gene at codon 200 in A. lumbricoides. We developed a technique based on an amplification refractory mutation system (ARMS-PCR) for the analysis of 854 single A. lumbricoides eggs collected from 68 human stool samples from seven Brazilian states. We detected the mutation in codon 200 at a frequency of 0.5% (4/854). This is the first report of this mutation in A. lumbricoides. Although the observed frequency is low, its presence indicates that these parasite populations have the potential to develop high levels of resistance in the future. The methodology proposed here provides a powerful tool to screen for the emergence of anthelmintic resistance mutations in parasitic nematode populations.
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