ArticlePLoS biology2019
Mechanically resolved imaging of bacteria using expansion microscopy.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
- Thrombin-dependent nanoscale architecture and fibrinolytic remodeling of hydrated fibrin clots revealed by expansion microscopy.Journal of thrombosis and haemostasis : JTH · 2026Article
- Fast, StreamlinedChemical & biomedical imaging · 2026Article
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Plants in the expansion era: recent advances in expansion microscopy in plant systems.Journal of experimental botany · 2025Review
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Highly multiplexed spatial transcriptomics in bacteria.Science (New York, N.Y.) · 2025Article
- Microbiome in cancer metastasis: biological insights and emerging spatial omics methods.Frontiers in cellular and infection microbiology · 2025Review
- Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Highly Multiplexed Spatial Transcriptomics in Bacteria.bioRxiv : the preprint server for biology · 2024Article
- Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy.bioRxiv : the preprint server for biology · 2024Article
- Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.Nature communications · 2024Article
- Emerging tools for uncovering genetic and transcriptomic heterogeneities in bacteria.Biophysical reviews · 2024Review
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Expansion microscopy applied to mono- and dual-species biofilms.NPJ biofilms and microbiomes · 2023Article
- MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Ultrafast and long-range coordination of wound responses is essential for whole-body regeneration.bioRxiv : the preprint server for biology · 2023Article
- MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues.Research square · 2023Article
- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.Methods in molecular biology (Clifton, N.J.) · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Imaging dense and diverse microbial communities has broad applications in basic microbiology and medicine, but remains a grand challenge due to the fact that many species adopt similar morphologies. While prior studies have relied on techniques involving spectral labeling, we have developed an expansion microscopy method (μExM) in which bacterial cells are physically expanded prior to imaging. We find that expansion patterns depend on the structural and mechanical properties of the cell wall, which vary across species and conditions. We use this phenomenon as a quantitative and sensitive phenotypic imaging contrast orthogonal to spectral separation to resolve bacterial cells of different species or in distinct physiological states. Focusing on host-microbe interactions that are difficult to quantify through fluorescence alone, we demonstrate the ability of μExM to distinguish species through an in vitro defined community of human gut commensals and in vivo imaging of a model gut microbiota, and to sensitively detect cell-envelope damage caused by antibiotics or previously unrecognized cell-to-cell phenotypic heterogeneity among pathogenic bacteria as they infect macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.