Evidence map›Paper›PMID 31620475›Full record

ArticleStem cell investigation2019

Isolation of umbilical cord mesenchymal stem cells using human blood derivatives accompanied with explant method.

Ghmkin Hassan, Issam Kasem, Reham Antaki, Mohammad Bahjat Mohammad, Ranad AlKadry, Majd Aljamali

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Article in Stem cell investigation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ghmkin HassanDepartment of Microbiology and Biochemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.
Issam KasemNational Commission for Biotechnology (NCBT), Damascus, Syria.
Reham AntakiNational Commission for Biotechnology (NCBT), Damascus, Syria.
Mohammad Bahjat MohammadNational Commission for Biotechnology (NCBT), Damascus, Syria.
Ranad AlKadryNational Commission for Biotechnology (NCBT), Damascus, Syria.
Majd AljamaliDepartment of Microbiology and Biochemistry, Faculty of Pharmacy, Damascus University, Damascus, Syria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) are multipotent cells which can be isolated from many sources including umbilical cord. Isolation protocols are depended on either explant or enzymatic methods. Although fetal bovine serum (FBS) is used as a supplement in isolation and expansion of MSCs, human blood derivatives such as cord blood serum (CBS) and platelet lysate (PL) are attractive substitutes of FBS which overcome impediments of using FBS in clinical setups.

methodsHere we compared the effect of using CBS, PL and FBS supplemented media in isolation of umbilical cord tissue derived MSCs by using explant method. To do that we cultured umbilical cord tissue explant in either CBS or PL or FBS supplemented media. Isolated cells were quantified, their morphology was assessed and Cells in passage 3 were characterized based on their immunophenotyping and their potential for differentiation into adipocytes and osteocytes. Moreover, proliferation of cells was assessed by crystal violet staining.

resultsAll the three media succeeded to isolate MSCs and maintain their stemness characteristics. However, the highest number of isolated cells were obtained using CBS, ~10-fold more than FBS, while the number of isolated cells obtained using PL was ~2-fold more than FBS. Moreover, crystal violet showed that both PL and CBS promote proliferation of MSCs more than FBS.

conclusionsOur data suggest that, although all supplements maintain stemness characteristics of MSCs when used to isolate those cells by explant method, using human blood derived supplements is more effective than FBS. In the same context CBS is more effective than PL.

Indexed as

cord blood serumfetal bovine serum (FBS)isolation of stem cellsMesenchymal stem cells (MSCs)platelet lysate

Identifiers

PMID31620475
PMCPMC6789207

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.