ArticleFrontiers in plant science2019
Factors Affecting Targeted Sequencing of 353 Nuclear Genes From Herbarium Specimens Spanning the Diversity of Angiosperms.
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed.
- Historical Plant Embryos as Alternative Sources of Ancient DNA for Whole Genome Sequencing.Molecular ecology resources · 2026Article
- Simultaneous Inference of Flora-Wide Nuclear and Plastid Phylogenies Using Angiosperms353 Sequence Capture Data.Molecular ecology resources · 2026Article
- BarnebyLives: An R package to create herbarium specimen labels and clean spreadsheets.Applications in plant sciences · 2026Article
- Contrasting Dispersal Histories Shape Distinct Evolutionary Trajectories Between Malesian and PantropicalEcology and evolution · 2026Article
- Enrichment Probe Sets Combining Universal and Lineage-Specific Targets Help Resolve Recalcitrant Lineages.Molecular ecology resources · 2026Article
- Whole genome sequencing of historical specimens from the world's largest fungal collection yields high-quality assemblies.The New phytologist · 2026Article
- Seeing herbaria in a new light: leaf reflectance spectroscopy unlocks trait and classification modeling in plant biodiversity collections.The New phytologist · 2026Article
- Nuclear phylogenomic analysis of Allium (Amaryllidaceae) species from Kazakhstan reveals incomplete lineage sorting and gene flow.BMC plant biology · 2026Article
- Genomic elucidation of hybridization between Liberica and excelsa coffee and its implications for coffee crop development.Scientific reports · 2026Article
- Patterns of Interploidy Admixture in Polyploid Complexes: Insights From Thymus Sect. Mastichina (Lamiaceae).Molecular ecology · 2026Article
- Short-read genome skimming enables molecular barcoding of old myxomycete collections.IMA fungus · 2026Article
- Urticaceae542 as a novel probe set combining universal and lineage-specific loci for phylogenomics of Urticaceae.Scientific reports · 2025Article
- UnFATE: A Comprehensive Probe Set and Bioinformatics Pipeline for Phylogeny Reconstruction and Multilocus Barcoding of Filamentous Ascomycetes (Ascomycota, Pezizomycotina).Systematic biology · 2025Article
- Genomic data define species delimitation in Liberica coffee with implications for crop development and conservation.Nature plants · 2025Article
- Conservation Implications for the Iberian Narrow EndemicEcology and evolution · 2025Article
- Integrating datasets from herbarium specimens and images to treat a Neotropical myrtle species complex.Annals of botany · 2025Article
- Exploring Structural Plastome Evolution in Asterales: Insights from Off-Target Hybrid Enrichment Data on the Small Single-Copy Region.Journal of molecular evolution · 2025Article
- Guidelines for the effective and ethical sampling of herbaria.Nature ecology & evolution · 2025Review
- Phylogenomics and classification of Cactaceae based on hundreds of nuclear genes.Plant systematics and evolution = Entwicklungsgeschichte und Systematik der Pflanzen · 2025Article
- Phylogenomic study ofFrontiers in plant science · 2025Article
11 more citing papers are in PubMed but not listed here.
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20 authors.
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Abstract
The world's herbaria collectively house millions of diverse plant specimens, including endangered or extinct species and type specimens. Unlocking genetic data from the typically highly degraded DNA obtained from herbarium specimens was difficult until the arrival of high-throughput sequencing approaches, which can be applied to low quantities of severely fragmented DNA. Target enrichment involves using short molecular probes that hybridise and capture genomic regions of interest for high-throughput sequencing. In this study on herbariomics, we used this targeted sequencing approach and the Angiosperms353 universal probe set to recover up to 351 nuclear genes from 435 herbarium specimens that are up to 204 years old and span the breadth of angiosperm diversity. We show that on average 207 genes were successfully retrieved from herbarium specimens, although the mean number of genes retrieved and target enrichment efficiency is significantly higher for silica gel-dried specimens. Forty-seven target nuclear genes were recovered from a herbarium specimen of the critically endangered St Helena boxwood,
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