ArticleNature communications2019
Nanosecond photochemically promoted click chemistry for enhanced neuropeptide visualization and rapid protein labeling.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Chemical Derivatization Strategies for Expanding Small Biomolecule Analysis by MALDI-MS.Metabolites · 2026Review
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Decoding Neuropeptide Complexity: Advancing Neurobiological Insights from Invertebrates to Vertebrates through Evolutionary Perspectives.ACS chemical neuroscience · 2025Review
- Polyamine Metabolism in Optic Nerve: Spatial Metabolomics with AP/MALDI Mass Spectrometry Imaging.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Mass spectrometry imaging: the rise of spatially resolved single-cell omics.Nature methods · 2023Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus.Journal of visualized experiments : JoVE · 2022Article
- Nanosecond Photochemical Reaction for Enhanced Identification, Quantification, and Visualization of Primary Amine-Containing Metabolites by MALDI-Mass Spectrometry.Analytical chemistry · 2022Article
- ADVANCES IN HIGH-RESOLUTION MALDI MASS SPECTROMETRY FOR NEUROBIOLOGY.Mass spectrometry reviews · 2022Review
- Nanosecond Photochemical Reaction (nsPCR) for Enhanced Mass Spectrometric Identification, Quantification, and Visualization of Metabolites and Neuropeptides.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recent developments of novel matrices and on-tissue chemical derivatization reagents for MALDI-MSI.Analytical and bioanalytical chemistry · 2021Review
- Complementary neuropeptide detection in crustacean brain by mass spectrometry imaging using formalin and alternative aqueous tissue washes.Analytical and bioanalytical chemistry · 2021Article
- [Methods and applications of single-cell proteomics analysis based on mass spectrometry].Se pu = Chinese journal of chromatography · 2021Review
- Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements.Current protein & peptide science · 2021Review
- Insight to Functional Conformation and Noncovalent Interactions of Protein-Protein Assembly Using MALDI Mass Spectrometry.Molecules (Basel, Switzerland) · 2020Review
- Signature-Ion-Triggered Mass Spectrometry Approach Enabled Discovery of N- and O-Linked Glycosylated Neuropeptides in the Crustacean Nervous System.Journal of proteome research · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Comprehensive protein identification and concomitant structural probing of proteins are of great biological significance. However, this is challenging to accomplish simultaneously in one confined space. Here, we develop a nanosecond photochemical reaction (nsPCR)-based click chemistry, capable of structural probing of proteins and enhancing their identifications through on-demand removal of surrounding matrices within nanoseconds. The nsPCR is initiated using a photoactive compound, 2-nitrobenzaldehyde (NBA), and is examined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Benefiting from the on-demand matrix-removal effect, this nsPCR strategy enables enhanced neuropeptide identification and visualization from complex tissue samples such as mouse brain tissue. The design shows great promise for structural probing of proteins up to 155 kDa due to the exclusive accessibility of nsPCR to primary amine groups, as demonstrated by its general applicability using a series of proteins with various lysine residues from multiple sample sources, with accumulated labeling efficiencies greater than 90%.
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