ArticleJournal of clinical microbiology2020
Point-Counterpoint: Should We Be Performing Metagenomic Next-Generation Sequencing for Infectious Disease Diagnosis in the Clinical Laboratory?
Article in Journal of clinical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 101 citations in OpenAlex.
- Metagenomic sequencing in intracranial pyogenic infections: limitations in viability and implications for antimicrobial decision-making.Annals of medicine and surgery (2012) · 2026Article
- Diagnostic challenges and lessons learned of Guillain-Barré syndrome mimicking central nervous system infection - a case report.BMC neurology · 2026Article
- Improving the Precision of Etiological Diagnosis in Bacterial Infections Using Molecular Technologies: A Comparative Analysis of Platforms, AI Integration, and Point-of-Care Deployment.International journal of molecular sciences · 2026Review
- Notes regarding the next era for theJournal of clinical microbiology · 2026Article
- Diagnostic performance and clinical utility of metagenomic next-generation sequencing in suspected pulmonary infections: a comparative study stratified by immune status.Frontiers in cellular and infection microbiology · 2026Article
- The diagnostic value of fine-needle aspiration cytology in the early diagnosis of pulmonary cryptococcosis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- The laboratory billing process and its applications to molecular microbiology testing: guidance for laboratorians.Journal of clinical microbiology · 2025Review
- Academic and clinical perspectives of metagenome sequencing as a diagnostic tool for infectious disease: an interpretive phenomenological study.BMC infectious diseases · 2025Article
- Metagenomics: a new frontier for routine pathology testing of gastrointestinal pathogens.Gut pathogens · 2025Article
- Uniting disciplines against antimicrobial resistance (AMR): highlights from a multidisciplinary inaugural AMR summit.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2025Review
- A retrospective observational study of mNGS test utilization to examine the role of diagnostic stewardship at two academic medical centers.Journal of clinical microbiology · 2024Observational
- Article
- Clinical evaluation of cell-free and cellular metagenomic next-generation sequencing of infected body fluids.Journal of advanced research · 2024Article
- Epstein-Barr virus (EBV) induced pneumonitis in a patient with breast cancer receiving neoadjuvant chemotherapy: A case report.Respiratory medicine case reports · 2023Article
- Clinical metagenomics-challenges and future prospects.Frontiers in microbiology · 2023Review
- Case report: The clinical utility of metagenomic next-generation sequencing in mucormycosis diagnosis caused by fatalFrontiers in pediatrics · 2023Article
- Article
- Detection, prevention and treatment of COVID-19 and opportunities for nanobiotechnology.View (Beijing, China) · 2022Review
- Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections.Journal of advanced research · 2022Review
- Diagnostic assay and technology advancement for detecting SARS-CoV-2 infections causing the COVID-19 pandemic.Analytical and bioanalytical chemistry · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
INTRODUCTIONWith established applications of next-generation sequencing in inherited diseases and oncology, clinical laboratories are evaluating the use of metagenomics for identification of infectious agents directly from patient samples, to aid in the diagnosis of infections. Metagenomic next-generation sequencing for infectious diseases promises an unbiased approach to detection of microbes that does not depend on growth in culture or the targeting of specific pathogens. However, the issues of contamination, interpretation of results, selection of databases used for analysis, and prediction of antimicrobial susceptibilities from sequencing data remain challenges. In this Point-Counterpoint, Steve Miller and Charles Chiu discuss the pros of using direct metagenomic sequencing, while Kyle Rodino and Melissa Miller argue for the use of caution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.