ArticleJournal of clinical microbiology2019
Impact of Fragmentation on Commutability of Epstein-Barr Virus and Cytomegalovirus Quantitative Standards.
Article in Journal of clinical microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Cytomegalovirus Viral Load Threshold to Guide Preemptive Therapy in Hematopoietic Cell Transplant Recipients: Correlation With Cytomegalovirus Disease.The Journal of infectious diseases · 2024Pooled it
- Evaluation of Microbiologics CMV verification panel for the quantitation of CMV DNA.Journal of clinical microbiology · 2026Article
- Intraspecific Variations and Characteristics of Clinical, Physiological, and Antifungal Susceptibility Profiles of Trichophyton rubrum in Southwest China.Mycopathologia · 2026Article
- Inter-laboratory variability in cytomegalovirus DNA quantification: implications for standardization and clinical monitoring.Journal of clinical microbiology · 2025Article
- Microbial evaluation of Melaleuca alternifolia essential oil for its antifungal activity against Trichophyton violaceum and its synergistic effects with ITZ and KTZ.Archives of dermatological research · 2025Article
- The impact of FDA-cleared molecular solutions for BK polyomavirus quantitation.Journal of clinical microbiology · 2025Review
- [Refractory or resistant cytomegalovirus infections after hematopoietic stem cell transplantation: diagnosis and management].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2024Review
- Performance of the cobas EBV and cobas BKV assays: multi-site comparison of standardized quantitation.Journal of clinical microbiology · 2024Article
- Analytical and Clinical Performance of the NeuMoDx™ Platform for Cytomegalovirus and Epstein-Barr Virus Viral Load Testing.Viruses · 2024Article
- Digital PCR to Measure SARS-CoV-2 RNA, Variants, and Outcomes in Youth.Journal of the Pediatric Infectious Diseases Society · 2023Article
- Advances and Challenges in Cytomegalovirus Detection Methods for Liver Transplant Donors.Diagnostics (Basel, Switzerland) · 2023Review
- Approach to hematopoietic cell transplant candidates with respiratory viral detection.Frontiers in pediatrics · 2023Review
- Forty Years of Molecular Diagnostics for Infectious Diseases.Journal of clinical microbiology · 2022Review
- Prediction of Symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 Infection by Quantitative Digital Polymerase Chain Reaction Normalized to International Units.Open forum infectious diseases · 2022Article
- Matrix Matters: Assessment of Commutability among BK Virus Assays and Standards.Journal of clinical microbiology · 2022Article
- Management of Cytomegalovirus, Epstein-Barr Virus, and HIV Viral Load Quality Control Data Using Unity Real Time.Journal of clinical microbiology · 2022Article
- The Standardization and Control of Serology and Nucleic Acid Testing for Infectious Diseases.Clinical microbiology reviews · 2021Review
- Progress and Challenges in the Prevention, Diagnosis, and Management of Cytomegalovirus Infection in Transplantation.Clinical microbiology reviews · 2020Review
- Persistent Challenges of Interassay Variability in Transplant Viral Load Testing.Journal of clinical microbiology · 2020Review
- HERQ-9 Is a New Multiplex PCR for Differentiation and Quantification of All Nine Human Herpesviruses.mSphere · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Despite the adaptation of international standards, quantitative viral load testing of transplant-associated viruses continues to be limited by interlaboratory disagreement. Studies have suggested that this disagreement and the poor commutability of standards may, in some cases, be linked to amplicon size and the fragmentation of circulating viral DNA. We evaluated target fragmentation as a cause of noncommutability and pretest fragmentation of quantitative standards as a potential means of increasing commutability and interassay agreement. Forty-two cytomegalovirus (CMV)-positive and 41 Epstein-Barr virus (EBV)-positive plasma samples, together with two different quantitative standards for each virus, were tested as unknowns using 10 different quantitative PCR assays at 5 different laboratories. Standards were tested both intact and after intentional fragmentation by ultrasonication. Quantitative agreement between methods was assessed, together with commutability, using multiple statistical approaches. Most assays yielded results within 0.5 log
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