ArticleThe European respiratory journal2020
Transcriptional characterisation of human lung cells identifies novel mesenchymal lineage markers.
Article in The European respiratory journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- The transcriptional landscape of developing human trisomy 21 lungs.American journal of respiratory cell and molecular biology · 2026Article
- Spatial transcriptomics of developing human lungs defines cellular phenotypes associated with age, lineage and location.Scientific reports · 2026Article
- Article
- Gene-ius at Work: Decoding Cell States through Expression.American journal of respiratory cell and molecular biology · 2025Article
- Cell Population-resolved Multiomics Atlas of the Developing Lung.American journal of respiratory cell and molecular biology · 2025Article
- VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development.Frontiers in cell and developmental biology · 2025Article
- Article
- A single-cell atlas of lung homeostasis reveals dynamic changes during development and aging.Communications biology · 2024Article
- Association of Fetal Lung Development Disorders with Adult Diseases: A Comprehensive Review.Journal of personalized medicine · 2024Review
- Single-Cell Transcriptomic Profiling Identifies Molecular Phenotypes of Newborn Human Lung Cells.Genes · 2024Article
- Hyperoxia prevents the dynamic neonatal increases in lung mesenchymal cell diversity.Scientific reports · 2024Article
- Single-Cell Profiling of Premature Neonate Airways Reveals a Continuum of Myeloid Differentiation.American journal of respiratory cell and molecular biology · 2023Article
- Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung.NPJ Regenerative medicine · 2023Article
- Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.The Journal of allergy and clinical immunology · 2023Article
- The lung mesenchyme in development, regeneration, and fibrosis.The Journal of clinical investigation · 2023Review
- Regionally distinct progenitor cells in the lower airway give rise to neuroendocrine and multiciliated cells in the developing human lung.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Single-cell RNA sequencing reveals the developmental program underlying proximal-distal patterning of the human lung at the embryonic stage.Cell research · 2023Article
- Perinatal origins of bronchopulmonary dysplasia-deciphering normal and impaired lung development cell by cell.Molecular and cellular pediatrics · 2023Review
- Review
- FGF18 promotes human lung branching morphogenesis through regulating mesenchymal progenitor cells.American journal of physiology. Lung cellular and molecular physiology · 2023Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
rationaleThe lung mesenchyme gives rise to multiple distinct lineages of cells in the mature respiratory system, including smooth muscle cells of the airway and vasculature. However, a thorough understanding of the specification and mesenchymal cell diversity in the human lung is lacking.
methodsWe completed single-cell RNA sequencing analysis of fetal human lung tissues. Canonical correlation analysis, clustering, cluster marker gene identification and t-distributed stochastic neighbour embedding representation was performed in Seurat. Cell populations were annotated using ToppFun. Immunohistochemistry and
resultsWe identified molecularly distinct populations representing "committed" fetal human lung endothelial cells, pericytes and smooth muscle cells. Early endothelial lineages expressed "classic" endothelial cell markers (platelet endothelial cell adhesion molecule/CD31 and claudin 5), while pericytes expressed platelet-derived growth factor receptor-β, Thy-1 membrane glycoprotein and basement membrane molecules (collagen IV, laminin and proteoglycans). We observed a large population of "nonspecific" human lung mesenchymal progenitor cells characterised by expression of collagen I and multiple elastin fibre genes (
conclusionOur data suggest that specification of distinct airway and vascular smooth muscle cell phenotypes is established early in development and can be identified using the markers we provide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.