ArticleParasites & vectors2019
Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
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- Methanotroph (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Functional feed ingredients modulate the immune response of RTgutGC cells to LPS-induced inflammation.Frontiers in immunology · 2025Article
- First insight of the genome-wide association study and genomic prediction into enteritis disease (Frontiers in immunology · 2024Article
- An Antioxidant Supplement Function Exploration: Rescue of Intestinal Structure Injury by Mannan Oligosaccharides afterAntioxidants (Basel, Switzerland) · 2022Article
- Dysregulation of Intestinal Physiology by Aflatoxicosis in the Gilthead Seabream (Frontiers in physiology · 2021Article
- Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare.Frontiers in veterinary science · 2021Review
- Natural Feed Additives Modulate Immunity and Mitigate Infection withPathogens (Basel, Switzerland) · 2020Article
- Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream.Microbiome · 2020Article
- Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish.Microorganisms · 2020Article
- Passive Immunization Delays Disease Outcome in Gilthead Sea Bream Infected WithFrontiers in immunology · 2020Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIn the animal production sector, enteritis is responsible for serious economic losses, and intestinal parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efficiency. The effect of enteric parasites on the gut function of teleost fish, which represent the most ancient bony vertebrates, is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced marketability and increased mortality.
methodsThis study aimed to outline the gut failure in this fish-parasite model using a multifaceted approach and to find and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized and non-parasitized fish by immunohistochemistry with specific markers for cellular adhesion (E-cadherin) and tight junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electrophysiology (Ussing chambers). Serum samples from parasitized and non-parasitized fish were analyzed using non-targeted metabolomics and some significantly altered metabolites were selected to be validated using commercial kits.
resultsThe immunodetection of Tjp1 and Cldn3 was significantly lower in the intestine of parasitized fish, while no strong differences were found in E-cadherin. Parasitized fish showed a significant increase in paracellular uptake measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in infected animals, which showed a diarrheic profile. Serum metabolomics revealed 3702 ions, from which the differential expression of 20 identified compounds significantly separated control from infected groups in multivariate analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identified as relevant and validated with commercial kits.
conclusionsThe results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metabolomic profile of absorption dysfunction and anorexia, which further outline the pathophysiological effects of E. leei.
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