Evidence map›Paper›PMID 31619262›Full record

ArticleVirology journal2019

Combining HPV DNA load with p16/Ki-67 staining to detect cervical precancerous lesions and predict the progression of CIN1-2 lesions.

Yuejie Li, Jie Liu, Li Gong, Xingwang Sun, Wenbo Long

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yuejie LiPathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street in Sichuan province Luzhou City Jiangyang District No. 25, Sichuan, China.
Jie LiuPathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street in Sichuan province Luzhou City Jiangyang District No. 25, Sichuan, China.
Li GongPathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street in Sichuan province Luzhou City Jiangyang District No. 25, Sichuan, China.
Xingwang SunPathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street in Sichuan province Luzhou City Jiangyang District No. 25, Sichuan, China.
Wenbo LongPathology Department of the First Affiliated Hospital, Southwest Medical University, Taiping Street in Sichuan province Luzhou City Jiangyang District No. 25, Sichuan, China. wenbolong@swmu.edu.cn.ORCID 0000-0002-9647-685X
Southwest Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman Papilloma Virus (HPV) DNA tests are highly sensitive and can triage women with mild lesions, improving the prognosis and diagnosis of cervical lesions. However, additional efficient strategies should be developed to improve the specificity of these tests.

methodsThis study aimed to evaluate the clinical value of HPV DNA load in improving the diagnosis and prognosis of cervical lesions by p16/Ki-67 testing. Histological samples were collected from 350 women with HR-HPV genotyping and analyzed by qRT-PCR. Immunohistochemical staining was used to assess p16 and Ki-67 expression and clinical performance characteristics were calculated.

resultsOf the cases, 271 had detectable HR-HPV infection, in which HPV-16 was most prevalent (52.0%), followed by HPV-58 (22.5%). P16/Ki-67-positivity increased with histological severity but not for HR-HPV infection. Amongst the 13 HR-HPV genotypes, only HPV-16 (P = 0.016) and HPV-58 (P = 0.004) viral loads significantly correlated with lesion severity. The P16/Ki-67/HPV DNA load co-test indicated an increased sensitivity for the detection of cervical intraepithelial neoplasia (CIN) lesions compared to p16/Ki-67 staining in HPV-16 and/or 58 positive cases. Viral load did not improve the sensitivity of p16/Ki-67 co-test in non-HPV-16 or 58 positive cases. The clinical performance of the p16/Ki-67/HPV DNA load co-test was limited for the prediction of the outcome of CIN1 lesions. However, amongst the 12 HPV-16 and/or 58 positive CIN2 cases in which return visit results were obtained, the behavior of the lesions could be predicted, with a sensitivity, specificity, positive prediction rate (PPV), and negative prediction rate (NPV) of 0.667, 1, 1 and 0.5, respectively.

conclusionCombination of the assessment of HPV DNA load with the intensity of p16 and Ki-67 staining could increase the sensitivity of CIN lesion diagnosis and predict the outcome of CIN2 in patients with a HPV-16 and/or 58 infection.

Indexed as

AdultAgedBiomarkers, TumorCyclin-Dependent Kinase Inhibitor p16Disease ProgressionDNA, ViralFemaleGenotypeHumansKi-67 AntigenMiddle AgedPapillomaviridaePapillomavirus InfectionsPrecancerous ConditionsPrognosisSensitivity and SpecificityBiomarkers, TumorCDKN2A protein, humanCyclin-Dependent Kinase Inhibitor p16DNA, ViralKi-67 AntigenDiagnosis and prognosisHPV DNA loadImmunohistochemistryp16/Ki-67/HPV DNA load co-test

Identifiers

PMID31619262
PMCPMC6794830
OpenAlexW2980887989

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.