Observational studyBMC microbiology2019
Characterization of Salmonella Dublin isolated from bovine and human hosts.
Observational study in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Invasive non-typhoidal Salmonella infections in China (1961-2024): a retrospective systematic analysis of multicentre case reports.Journal of global health · 2026Pooled it
- Genomic characterization ofVirulence · 2026Article
- Cross-species genomic analysis ofOne health (Amsterdam, Netherlands) · 2026Article
- Article
- Article
- Dynamics of Salmonella Dublin infection and antimicrobial resistance in a dairy herd endemic to salmonellosis.PloS one · 2025Article
- The potential of using bacteriophages targetingFrontiers in microbiology · 2025Review
- Identification and Characterization ofPHAGE (New Rochelle, N.Y.) · 2024Article
- Article
- Genomic Profiling of Antimicrobial Resistance Genes in ClinicalAntibiotics (Basel, Switzerland) · 2024Article
- Multi-locus sequence typing, antimicrobials resistance and virulence profiles of Salmonella enterica isolated from bovine carcasses in Minas Gerais state, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Molecular Characteristics ofInfection and drug resistance · 2024Article
- Prevalence and Characterization ofPathogens (Basel, Switzerland) · 2023Article
- Epidemiological Analysis on the Occurrence ofMicroorganisms · 2023Article
- Phenotypic and genotypic survey of antibiotic resistance inFrontiers in veterinary science · 2023Article
- Clonal relation betweenFrontiers in veterinary science · 2023Article
- Review:Frontiers in veterinary science · 2023Review
- Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella.Microbiology spectrum · 2022Article
- Genomic Characterization of Antimicrobial-Resistant Salmonella enterica in Duck, Chicken, and Pig Farms and Retail Markets in Eastern China.Microbiology spectrum · 2022Article
- A retrospective and regional approach assessing the genomic diversity ofNAR genomics and bioinformatics · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella enterica subsp. enterica serovar Dublin (S. Dublin), a cattle adapted serovar causes enteritis, and systemic disease in bovines. The invasive index of this serovar far exceeds that of the other serovars and human infections often present as fatal or highly resistant infections. In this, observational study, phenotypic properties of human and bovine-derived isolates of S. Dublin along with antibiogram of common antimicrobials were evaluated. The multiplex PCR confirmed isolates were genotyped using 7-gene legacy MLST. MIC assay was done by broth microdilution method. Previously published protocols were used to assess the motility, biofilm formation and morphotype. Vi antigen was agglutinated using commercial antiserum. Caenorhabditis elegans infection model was used to evaluate the virulence potiential. Phenotyping experiments were done in duplicates while virulence assay was done in triplicates. Whole-genome sequencing was used to predict the genes responsible for acquired resistance and a genotype-phenotype comparison was made.
resultsWe evaluated 96 bovine and 10 human isolates in this study. All the isolates belonged to ST10 in eBG53 and were negative for Vi-antigen. The swarming motility, biofilm formation and morphotype were variable in the isolates of both groups. Resistance to sulfamethoxazole, ampicillin, chloramphenicol, tetracycline was > 90% in animal isolates whereas resistance to sulfamethoxazole was > 70% in human isolates. MDR was also higher in animal isolates. Human isolates were significantly (P < 0.0001) more virulent than animal isolates on C. elegans infection model. The genomic comparison based on the core SNPs showed a high degree of homogeneity between the isolates. The carriage of IncA/C2 plasmid was seen as a typical feature of isolates from the bovine hosts.
conclusionHuman isolates showed more diversity in the phenotypic assays. Animal isolates showed a higher degree of antimicrobial resistance with greater MDR but human isolates formed more biofilm and had greater swarming motility as well as increased virulence to the nematode C. elegans. The carriage of IncA/C2 plasmid could contribute to the distinguishing feature of the bovine isolates. The tandem use of genotypic-phenotypic assays improves the understanding of diversity and differential behaviour of the same serovar from unrelated host sources.
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