ArticleChemistry (Weinheim an der Bergstrasse, Germany)2019
3D-Printed Phenacrylate Decarboxylase Flow Reactors for the Chemoenzymatic Synthesis of 4-Hydroxystilbene.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Additive Manufacturing in Organic Chemistry: From Synthesis to Sustainable Process Design.International journal of molecular sciences · 2026Review
- Integrating biocatalysis with continuous flow: current status, challenges, and future perspectives.Journal of advanced research · 2026Review
- Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes.ChemSusChem · 2025Review
- 3D Printing in Biocatalysis and Biosensing: From General Concepts to Practical Applications.Chemistry, an Asian journal · 2024Review
- Stability Increase of Phenolic Acid Decarboxylase by a Combination of Protein and Solvent Engineering Unlocks Applications at Elevated Temperatures.ACS sustainable chemistry & engineering · 2024Article
- Advances in 3D Gel Printing for Enzyme Immobilization.Gels (Basel, Switzerland) · 2022Review
- A Magnetosome-Based Platform for Flow Biocatalysis.ACS applied materials & interfaces · 2022Article
- Emerging 3D Printing Strategies for Enzyme Immobilization: Materials, Methods, and Applications.ACS omega · 2022Review
- Systematic evaluation of agarose- and agar-based bioinks for extrusion-based bioprinting of enzymatically active hydrogels.Frontiers in bioengineering and biotechnology · 2022Article
- Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds.International journal of molecular sciences · 2021Review
- Immobilization of β-Galactosidase by Encapsulation of Enzyme-Conjugated Polymer Nanoparticles Inside Hydrogel Microparticles.Frontiers in bioengineering and biotechnology · 2021Article
- Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications.Molecules (Basel, Switzerland) · 2020Review
- 3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions.Frontiers in bioengineering and biotechnology · 2020Article
- 3D-Printed Phenacrylate Decarboxylase Flow Reactors for the Chemoenzymatic Synthesis of 4-Hydroxystilbene.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- A Phenolic Acid Decarboxylase-Based All-Enzyme Hydrogel for Flow Reactor Technology.Micromachines · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Continuous flow systems for chemical synthesis are becoming a major focus in organic chemistry and there is a growing interest in the integration of biocatalysts due to their high regio- and stereoselectivity. Methods established for 3D bioprinting enable the fast and simple production of agarose-based modules for biocatalytic reactors if thermally stable enzymes are available. We report here on the characterization of four different cofactor-free phenacrylate decarboxylase enzymes suitable for the production of 4-vinylphenol and test their applicability for the encapsulation and direct 3D printing of disk-shaped agarose-based modules that can be used for compartmentalized flow microreactors. Using the most active and stable phenacrylate decarboxylase from Enterobacter spec. in a setup with four parallel reactors and a subsequent palladium(II) acetate-catalysed Heck reaction, 4-hydroxystilbene was synthesized from p-coumaric acid with a total yield of 14.7 % on a milligram scale. We believe that, due to the convenient direct immobilization of any thermostable enzyme and straightforward tuning of the reaction sequence by stacking of modules with different catalytic activities, this simple process will facilitate the establishment and use of cascade reactions and will therefore be of great advantage for many research approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.