ArticleJournal of veterinary internal medicine2019
Immunoreactive insulin stability in horses at risk of insulin dysregulation.
Article in Journal of veterinary internal medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Aleurone supplementation enhances the metabolic benefits of training in Standardbred mares: impacts on glucose-insulin dynamics and gut microbiome composition.Frontiers in physiology · 2025Article
- Effect of sirolimus on insulin dynamics in horses.Journal of veterinary internal medicine · 2023Article
- Changes Associated with the Peri-Ovulatory Period, Age and Pregnancy in ACTH, Cortisol, Glucose and Insulin Concentrations in Mares.Animals : an open access journal from MDPI · 2021Article
- Immunoreactive insulin stability in horses at risk of insulin dysregulation.Journal of veterinary internal medicine · 2019Article
- Effect of phenylbutazone administration on the enteroinsular axis in horses with insulin dysregulation.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundDiseases associated with insulin dysregulation (ID), such as equine metabolic syndrome and pituitary pars intermedia dysfunction, are of interest to practitioners because of their association with laminitis. Accurate insulin concentration assessment is critical in diagnosing and managing these diseases. HYPOTHESIS/
objectivesTo determine the effect of time, temperature, and collection tube type on insulin concentrations in horses at risk of ID. ANIMALS: Eight adult horses with body condition score >6/9.
methodsIn this prospective study, subjects underwent an infeed oral glucose test 2 hours before blood collection. Blood samples were divided into ethylenediaminetetraacetic acid, heparinized, or serum tubes and stored at 4 or 20°C. Tubes were centrifuged and analyzed for insulin by a chemiluminescent assay over 8 days. Changes in insulin concentrations were compared with a linear mixed effects model.
resultsAn overall effect of time, tube type and temperature was identified (P = .01, P = 0.001, and P = 0.001, respectively). Serum and heparinized samples had similar concentrations for 3 days at 20°C and 8 days at 4°C; however, after 3 days at 20°C, heparinized samples had significantly higher insulin concentrations (P = .004, P = .03, and P = .03 on consecutive days). Ethylenediaminetetraacetic acid samples had significantly lower insulin concentrations regardless of time and temperature (P = .001 for all comparisons). CONCLUSIONS AND CLINICAL IMPORTANCE: These results suggest an ideal protocol to determine insulin concentrations involves using serum or heparinized samples with analysis occurring within 3 days at 20°C or 8 days at 4°C.
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Registered trials
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