ArticleBiology of reproduction2020
Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy†.
Article in Biology of reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Comparative profiling of extracellular vesicles and miRNA cargo from in vivo- and in vitro-derived bovine embryos during blastulation and hatching.Journal of animal science and biotechnology · 2026Article
- When bovine life begins in a petri dish: transcriptomic insights from embryos to calves conceived in vitro.The Journal of reproduction and development · 2026Review
- Development of a formula for scoring competence of bovine embryos to sustain pregnancy.Biochemistry and biophysics reports · 2024Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero.Reproduction & fertility · 2023Article
- Colony stimulating factor 2 protects the preimplantation bovine embryo from heat shock.Zygote (Cambridge, England) · 2023Article
- Bioactive supplements influencing bovine in vitro embryo development.Journal of animal science · 2022Review
- Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.Scientific reports · 2022Article
- Cytokines That Serve as Embryokines in Cattle.Animals : an open access journal from MDPI · 2021Review
- Programming of postnatal phenotype caused by exposure of cultured embryos from Brahman cattle to colony-stimulating factor 2 and serum.Journal of animal science · 2021Article
- The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer.Metabolites · 2021Article
- The global gene expression outline of the bovine blastocyst: reflector of environmental conditions and predictor of developmental capacity.BMC genomics · 2021Article
- Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA.Biology of reproduction · 2021Article
- Metabolites Secreted by Bovine Embryos In Vitro Predict Pregnancies That the Recipient Plasma Metabolome Cannot, and Vice Versa.Metabolites · 2021Article
- An improved method for specific-target preamplification PCR analysis of single blastocysts useful for embryo sexing and high-throughput gene expression analysis.Journal of dairy science · 2021Article
- PGE2 Supplementation of Oocyte Culture Media Improves the Developmental and Cryotolerance Performance of Bovine Blastocysts Derived From a Serum-FreeFrontiers in cell and developmental biology · 2021Article
- Article
- Genes associated with survival of female bovine blastocysts produced in vivo.Cell and tissue research · 2020Article
- The incompletely fulfilled promise of embryo transfer in cattle-why aren't pregnancy rates greater and what can we do about it?Journal of animal science · 2020Review
- Actions of colony-stimulating factor 3 on the maturing oocyte and developing embryo in cattle.Journal of animal science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence to establish and maintain gestation. Embryos produced with X-sorted sperm were cultured from day 5 to day 7 in serum-free medium containing 10 ng/ml recombinant bovine colony-stimulating factor 2 (CSF2) or vehicle. The CSF2 was administered because this molecule can increase blastocyst competence for survival after embryo transfer. Blastocysts were harvested on day 7 of culture and manually bisected. One demi-embryo from a single blastocyst was transferred into a synchronized recipient and the other half was used for RNA-seq analysis. Using P < 0.01 and a fold change >2-fold or <0.5 fold as cutoffs, there were 617 differentially expressed genes (DEG) between embryos that survived to day 30 of gestation vs those that did not, 470 DEG between embryos that survived to day 60 and those that did not, 432 DEG between embryos that maintained pregnancy from day 30 to day 60 vs those where pregnancy failed after day 30, and 635 DEG regulated by CSF2. Pathways and ontologies in which DEG were overrepresented included many related to cellular responses to stress and cell survival. It was concluded that gene expression in the blastocyst is different between embryos that are competent to establish and maintain pregnancy vs those that are not. The relationship between expression of genes related to cell stress and subsequent embryonic survival probably reflects cellular perturbations caused by embryonic development taking place in the artificial environment associated with cell culture.
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