ArticleFrontiers in medicine2019
Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.
Article in Frontiers in medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- The complex diagnosis of post-dialysis fever: a case report and literature review of infective endocarditis in a dialysis patient.BMC nephrology · 2025Review
- Case report: The value of early application of mNGS technology in the diagnosis and treatment of severe Legionnaires' disease: reports of two cases with different outcomes.Frontiers in medicine · 2025Article
- Culture-Negative Endocarditis Complicated by Cerebral Abscesses Due to Streptococcus gordonii: A Diagnostic Odyssey.Cureus · 2024Article
- Performance of 16S rRNA Gene Next-Generation Sequencing and the Culture Method in the Detection of Bacteria in Clinical Specimens.Diagnostics (Basel, Switzerland) · 2024Article
- Metagenomics for the microbiological diagnosis of hospital-acquired pneumonia and ventilator-associated pneumonia (HAP/VAP) in intensive care unit (ICU): a proof-of-concept study.Respiratory research · 2023Article
- Endocarditis due to Aggregatibacter Segnis: a rare case report.BMC infectious diseases · 2023Article
- Review
- Blood Culture Negative Endocarditis: A Review of Laboratory Diagnostic Approaches.International journal of general medicine · 2023Review
- Application of metagenomic next-generation sequencing in patients with infective endocarditis.Frontiers in cellular and infection microbiology · 2023Article
- Identification of prosthetic joint infection byAccess microbiology · 2023Article
- Effect of bacterial DNA enrichment on detection and quantification of bacteria in an infected tissue model by metagenomic next-generation sequencing.ISME communications · 2022Article
- Pathogen Detection in Infective Endocarditis Using Targeted Metagenomics on Whole Blood and Plasma: a Prospective Pilot Study.Journal of clinical microbiology · 2022Article
- The First Case ofPathogens (Basel, Switzerland) · 2022Article
- Metagenomic Sequencing for Microbial DNA in Human Samples: Emerging Technological Advances.International journal of molecular sciences · 2022Review
- Native valve, prosthetic valve, and cardiac device-related infective endocarditis: A review and update on current innovative diagnostic and therapeutic strategies.Frontiers in cell and developmental biology · 2022Review
- Case Report: An Ulceration With a Stalactite Appearance on the Index Finger.Frontiers in medicine · 2022Article
- What Does 16S rRNA Gene-Targeted Next Generation Sequencing Contribute to the Study of Infective Endocarditis in Heart-Valve Tissue?Pathogens (Basel, Switzerland) · 2021Article
- Article
- Case Report: Metagenomic Next-Generation Sequencing in Diagnosis ofFrontiers in medicine · 2021Article
- Endocarditis caused byIDCases · 2021Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.