Evidence map›Paper›PMID 31616589›Full record

ArticlePeerJ2019

Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix).

Travis C Glenn, Todd W Pierson, Natalia J Bayona-Vásquez, Troy J Kieran, Sandra L Hoffberg, Jesse C Thomas Iv, Daniel E Lefever, John W Finger, Bei Gao, Xiaoming Bian and 17 more

Abstract read
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Travis C GlennDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Todd W PiersonDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Natalia J Bayona-VásquezDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Troy J KieranDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Sandra L HoffbergDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Jesse C Thomas IvDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Daniel E LefeverDepartment of Veterinary Biosciences and Diagnostic Imaging, University of Georgia, Athens, GA, United States of America.
John W FingerDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Bei GaoDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Xiaoming BianDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Swarnali LouhaInstitute of Bioinformatics, University of Georgia, Athens, GA, United States of America.
Ramya T KolliInterdisciplinary Toxicology Program, University of Georgia, Athens, GA, United States of America.
Kerin E BentleyDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Julie RushmoreSchool of Ecology & College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America.
Kelvin WongUS Environmental Protection Agency, Athens, GA, United States of America.
Timothy I ShawInstitute of Bioinformatics, University of Georgia, Athens, GA, United States of America.
Michael J RothrockU.S. National Poultry Research Center, USDA-ARS, Athens, GA, United States of America.
Anna M McKeeSouth Atlantic Water Science Center, U.S. Geological Survey, Norcross, GA, United States of America.
Tai L GuoDepartment of Veterinary Biosciences and Diagnostic Imaging, University of Georgia, Athens, GA, United States of America.
Rodney MauricioDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Marirosa MolinaUS Environmental Protection Agency, Athens, GA, United States of America.
Brian S CummingsInterdisciplinary Toxicology Program, University of Georgia, Athens, GA, United States of America.
Lawrence H LashDepartment of Pharmacology, Wayne State University, Detroit, MI, United States of America.
Kun LuDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Gregory S GilbertEnvironmental Studies Department, University of California, Santa Cruz, Santa Cruz, CA, United States of America.
Stephen P HubbellSmithsonian Tropical Research Institute, Balboa, Ancon, Panama.
Brant C FairclothDepartment of Biological Sciences and Museum of Natural Science, Louisiana State University, Baton Rouge, LA, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to full-length adapter sequences with a single identifying sequence or ligating adapters onto PCR products. In

Indexed as

Fusion primersHierarchical indexingInternal taggingLibrariesMiSeqMultiplexingNext generation sequencingPCRQuadruple indexing

Identifiers

PMID31616589
PMCPMC6791344

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.