Evidence map›Paper›PMID 31616583›Full record

ArticlePeerJ2019

Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD).

Natalia J Bayona-Vásquez, Travis C Glenn, Troy J Kieran, Todd W Pierson, Sandra L Hoffberg, Peter A Scott, Kerin E Bentley, John W Finger, Swarnali Louha, Nicholas Troendle and 3 more

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 177 citations in OpenAlex.

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  8. Population Genetics Reveals Insights IntoEcology and evolution · 2026
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  14. Low-coverage whole-genome sequencing facilitates accurate and cost-effective haplotype reconstruction in complex mouse crosses.Mammalian genome : official journal of the International Mammalian Genome Society · 2025
    Article
  15. Review
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  20. Article

4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 2 countries.

Natalia J Bayona-Vásquez *Department of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Travis C Glenn *Department of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Troy J KieranDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Todd W PiersonDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Sandra L HoffbergDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Peter A ScottDepartment of Biological Sciences, University of Alabama, Tuscaloosa, AL, United States of America.
Kerin E BentleyDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
John W FingerDepartment of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Swarnali LouhaInstitute of Bioinformatics, University of Georgia, Athens, GA, United States of America.
Nicholas TroendleDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Pindaro Diaz-JaimesUnidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Rodney MauricioDepartment of Genetics, University of Georgia, Athens, GA, United States of America.
Brant C FairclothDepartment of Biological Sciences and Museum of Natural Science, Louisiana State University, Baton Rouge, LA, United States of America.
University of Georgia · USUniversidad Nacional Autónoma de México · MXAuburn University · USColumbia University · USHouston Museum of Natural Science · USMidAmerica Nazarene University · USUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing methods expensive to initiate and/or difficult to implement consistently, especially because it is difficult to multiplex sufficient numbers of samples to fill entire sequencing lanes. Here, we introduce a low-cost and highly robust approach for the construction of dual-digest RADseq libraries that build on adapters and primers designed in

Indexed as

ddRADHiSeqIlluminaIn-line barcodesiTruMultiplexingNext generation sequencingNovaSeqReduced representation libraryRestriction enzyme

Identifiers

PMID31616583
PMCPMC6791345
OpenAlexW2979770188

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.