Evidence map›Paper›PMID 31615563›Full record

ArticleBMC cancer2019

CSB affected on the sensitivity of lung cancer cells to platinum-based drugs through the global decrease of let-7 and miR-29.

Zhenbang Yang, Chunling Liu, Hongjiao Wu, Yuning Xie, Hui Gao, Xuemei Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Structure and Function of SNM1 Family Nucleases.Advances in experimental medicine and biology · 2023
    Review
  5. Review
  6. Review
  7. MicroRNA-29b regulates the radiosensitivity of esophageal squamous cell carcinoma by regulating the BTG2-mediated cell cycle.Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al] · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Zhenbang YangInstitute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
Chunling LiuDepartment of Pathology, Affiliated Tangshan Renmin Hospital North China University of Science and Technology, Tangshan, China.
Hongjiao WuInstitute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
Yuning XieInstitute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
Hui GaoInstitute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
Xuemei ZhangInstitute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China. jyxuemei@gmail.com.ORCID http://orcid.org/0000-0003-0705-4231
North China University of Science and Technology · CN

Funding

Hebei high-level talent cultivation plan in University GCC2014050Key Project of Natural Science Foundation of Hebei province of China H2017209233National Natural Science Foundation of China 81272613
6 · The paper itself

Abstract

backgroundTranscription-coupled nucleotide excision repair (TC-NER) plays a prominent role in the removal of DNA adducts induced by platinum-based chemotherapy reagents. Cockayne syndrome protein B (CSB), the master sensor of TCR, is also involved in the platinum resistant. Let-7 and miR-29 binding sites are highly conserved in the proximal 3'UTR of CSB.

methodsWe conducted immunohistochemisty to examine the expression of CSB in NSCLC. To determine whether let-7 family and miR-29 family directly interact with the putative target sites in the 3'UTR of CSB, we used luciferase reporter gene analysis. To detect the sensitivity of non-small cell lung cancer (NSCLC) cells to platinum-based drugs, CCK analysis and apoptosis analysis were performed.

resultsWe found that let-7 and miR-29 negatively regulate the expression of CSB by directly targeting to the 3'UTR of CSB. The endogenous CSB expression could be suppressed by let-7 and miR-29 in lung cancer cells. The suppression of CSB activity by endogenous let-7 and miR-29 can be robustly reversed by their sponges. Down-regulation of CSB induced apoptosis and increased the sensitivity of NSCLC cells to cisplatin and carboplatin drugs. Let-7 and miR-29 directly effect on cisplatin and carboplatin sensitivity in NSCLC.

conclusionsIn conclusion, the platinum-based drug resistant of lung cancer cells may involve in the regulation of let-7 and miR-29 to CSB.

Indexed as

3' Untranslated RegionsA549 CellsAntineoplastic AgentsApoptosisBinding SitesCarboplatinCarcinoma, Non-Small-Cell LungCisplatinDNA HelicasesDNA RepairDNA Repair EnzymesDrug Resistance, NeoplasmGene Expression Regulation, NeoplasticHumansLung NeoplasmsMicroRNAs3' Untranslated RegionsAntineoplastic AgentsCarboplatinCisplatinDNA HelicasesDNA Repair EnzymesERCC6 protein, humanMicroRNAsMIRN29a microRNA, humanmirnlet7 microRNA, humanPoly-ADP-Ribose Binding ProteinsCSBLet-7Lung cancermiR-29Platinum

Identifiers

PMID31615563
PMCPMC6792260
OpenAlexW2980900685

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.