ArticleBMC plant biology2019
A modular steroid-inducible gene expression system for use in rice.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.Plant biotechnology journal · 2026Article
- Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses.Plant biotechnology journal · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- GOLDEN2-like1 is sufficient but not necessary for chloroplast biogenesis in mesophyll cells of CThe Plant journal : for cell and molecular biology · 2024Article
- Glyphosate-induced GhAG2 is involved in resistance to salt stress in cotton.Plant cell reports · 2022Article
- A procedure for Dex-induced gene transactivation in Arabidopsis ovules.Plant methods · 2022Article
- Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research.International journal of molecular sciences · 2022Review
- The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa).BMC plant biology · 2021Article
- The soybean GmNFY-B1 transcription factor positively regulates flowering in transgenic Arabidopsis.Molecular biology reports · 2021Article
- Mitigation of emerging implications of climate change on food production systems.Food research international (Ottawa, Ont.) · 2020Review
- A heat-shock inducible system for flexible gene expression in cereals.Plant methods · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundChemically inducible systems that provide both spatial and temporal control of gene expression are essential tools, with many applications in plant biology, yet they have not been extensively tested in monocotyledonous species.
resultsUsing Golden Gate modular cloning, we have created a monocot-optimized dexamethasone (DEX)-inducible pOp6/LhGR system and tested its efficacy in rice using the reporter enzyme β-glucuronidase (GUS). The system is tightly regulated and highly sensitive to DEX application, with 6 h of induction sufficient to induce high levels of GUS activity in transgenic callus. In seedlings, GUS activity was detectable in the root after in vitro application of just 0.01 μM DEX. However, transgenic plants manifested severe developmental perturbations when grown on higher concentrations of DEX. The direct cause of these growth defects is not known, but the rice genome contains sequences with high similarity to the LhGR target sequence lacO, suggesting non-specific activation of endogenous genes by DEX induction. These off-target effects can be minimized by quenching with isopropyl β-D-1-thiogalactopyranoside (IPTG).
conclusionsOur results demonstrate that the system is suitable for general use in rice, when the method of DEX application and relevant controls are tailored appropriately for each specific application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.