ArticleNature microbiology2019
BioID screen of Salmonella type 3 secreted effectors reveals host factors involved in vacuole positioning and stability during infection.
Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
- Proximity biotinylation at the host-Shigella interface reveals UFMylation as an antibacterial pathway.Nature communications · 2026Article
- Listeria promotes systemic infection by targeting the host deubiquitinase CYLD.Nature communications · 2026Article
- Type III secretion system chaperones: a helping hand for secretion.Microbiology (Reading, England) · 2026Review
- A conserved peptidase governs glucose homeostasis inbioRxiv : the preprint server for biology · 2026Article
- Emerging approaches for characterizing spatial and temporal dynamics of pathogen-induced organelle remodeling.Cell systems · 2026Review
- Parallel Genome-Wide CRISPR Screens Reveal SORL1 and ZFYVE19 as Sequential Host Determinants of Salmonella Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Intracellular Salmonella hijacks the mitochondrial citrate carrier to evade host oxidative defenses.Nature communications · 2025Article
- Proximity labelling reveals VPS13C as a regulator of Salmonella-containing vacuole fission.PLoS pathogens · 2025Article
- Proximity biotinylation at the host-bioRxiv : the preprint server for biology · 2025Article
- Characterizing host-microbe interactions with bacterial effector proteins using proximity-dependent biotin identification (BioID).Communications biology · 2025Review
- The interplay betweenCell insight · 2025Review
- MappingmBio · 2025Article
- Exploring Bacterial Surface Proteome Dynamics During Infection Using Proximity Labeling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC inmSphere · 2024Article
- Functional Divergence of the ParalogInternational journal of molecular sciences · 2024Review
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- Shift in vacuolar to cytosolic regime of infecting Salmonella from a dual proteome perspective.PLoS pathogens · 2023Article
- A host E3 ubiquitin ligase regulatesmLife · 2023Article
- Photo-ANA enables profiling of host-bacteria protein interactions during infection.Nature chemical biology · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Many bacterial pathogens express virulence proteins that are translocated into host cells (herein referred to as effectors), where they can interact with target proteins to manipulate host cell processes. These effector-host protein interactions are often dynamic and transient in nature, making them difficult to identify using traditional interaction-based methods. Here, we performed a systematic comparison between proximity-dependent biotin labelling (BioID) and immunoprecipitation coupled with mass spectrometry to investigate a series of Salmonella type 3 secreted effectors that manipulate host intracellular trafficking (SifA, PipB2, SseF, SseG and SopD2). Using BioID, we identified 632 candidate interactions with 381 unique human proteins, collectively enriched for roles in vesicular trafficking, cytoskeleton components and transport activities. From the subset of proteins exclusively identified by BioID, we report that SifA interacts with BLOC-2, a protein complex that regulates dynein motor activity. We demonstrate that the BLOC-2 complex is necessary for SifA-mediated positioning of Salmonella-containing vacuoles, and affects stability of the vacuoles during infection. Our study provides insight into the coordinated activities of Salmonella type 3 secreted effectors and demonstrates the utility of BioID as a powerful, complementary tool to characterize effector-host protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.