ArticleScientific reports2019
Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Antibiotic-Free Hypothermic Storage of Boar Semen at 5 °C with Next-Day On-Farm Cooling: Fertility and Biosafety Under Field Conditions.Antibiotics (Basel, Switzerland) · 2026Article
- Preliminary study of pregnancy rates and litter sizes following artificial insemination of boar spermatozoa prepared by colloid centrifugation and hypothermic storage.Scientific reports · 2025Article
- Photodynamic Inactivation of Bacteria in Boar Semen with Blue LED Light.Microorganisms · 2025Article
- Effect of Colloid Centrifugation and Cold Storage of Boar Semen at 4 °C on Bacterial Load and Sperm Quality.Antibiotics (Basel, Switzerland) · 2025Article
- Pre-cooling of boar semen before transport in a hot environment enhances biosafety.Frontiers in microbiology · 2025Article
- Artificial insemination of boar semen doses prepared with a low-density colloid under field conditions.Frontiers in veterinary science · 2025Article
- Assessment of Bacterial Contamination in Semen.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Effects of Different Antimicrobial Peptides (A-11 and AP19) on Isolated Bacteria from Fresh Boar Semen and Semen Quality during Storage at 18 °C.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Sperm quality andFrontiers in veterinary science · 2024Article
- Investigating the safety of antibiotics added to collared peccary (Animal reproduction · 2024Article
- Fertility after photodynamic inactivation of bacteria in extended boar semen.Frontiers in microbiology · 2024Article
- Validation of the volumetric flow cytometry for bovine sperm concentration.Scientific reports · 2023Article
- Bacteriospermia and its antimicrobial resistance in relation to boar sperm quality during short-term storage with or without antibiotics in a tropical environment.Porcine health management · 2023Article
- Storage of Extended Boar Semen at 5 °C Inhibits Growth of Multi-Drug ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Growth Dynamic and Threshold Values for Spermicidal Effects of Multidrug-Resistant Bacteria in Extended Boar Semen.Microorganisms · 2023Article
- N-thiocarboxyanhydrides, amino acid-derived enzyme-activated HBMC veterinary research · 2023Article
- The multidrug-resistantFrontiers in microbiology · 2023Article
- Storage of boar semen at 17°C without conventional antibiotics in an extender containing an organic bactericidal substance.Frontiers in veterinary science · 2023Article
- Bacteria and Boar Semen Storage: Progress and Challenges.Antibiotics (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of antibiotics (AB) in semen extenders as a potential contribution to the global antimicrobial resistance threat is emerging. Here, we establish an AB-free hypothermic preservation strategy for boar semen and investigate its impact on sperm function, microbial load and fertility after artificial insemination (AI). Spermatozoa (12 boars) preserved in AB-free AndroStar Premium extender at 5 °C maintained high motility, membrane integrity, and a low DNA-fragmentation index throughout 72 h storage and results did not significantly differ from controls stored at 17 °C in extender containing AB (p = 0.072). Likewise, kinetic response of spermatoza to the capacitation stimulus bicarbonate during 180 min incubation in Tyrode's medium did not differ from 17 °C-controls. In a competitive sperm oviduct binding assay, binding indices did not differ between semen stored for 72 h AB-free at 5 °C and 17 °C-controls (n = 6 boars). Bacterial load < 10
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