ArticleNature communications2019
Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Accurate prediction of oligonucleotide probe hybridization efficiency via a transformer-based model.BMC genomics · 2026Article
- Spatial fluorescence barcode by transiently luminescent DNA beads.Nature communications · 2025Article
- Design of mismatch closure for enhanced specificity in DNA strand displacement reactions.Nucleic acids research · 2025Article
- Methodology and application of multiplex PCR-dipstick DNA chromatography for the detection of eight respiratory bacterial pathogens.Frontiers in cellular and infection microbiology · 2025Article
- Advances in nucleic acid probe-based detection of gene point mutations: a review.Frontiers in chemistry · 2025Review
- Kinetic Proofreading Can Enhance Specificity in a Nonenzymatic DNA Strand Displacement Network.Journal of the American Chemical Society · 2024Article
- Heteromultivalency enables enhanced detection of nucleic acid mutations.Nature chemistry · 2024Article
- Beneficial and detrimental effects of non-specific binding during DNA hybridization.Biophysical journal · 2023Article
- A versatile and convenient tool for regulation of DNA strand displacement and post-modification on pre-fabricated DNA nanodevices.Nucleic acids research · 2023Article
- PAM-independent ultra-specific activation of CRISPR-Cas12a via sticky-end dsDNA.Nucleic acids research · 2022Article
- Ultrasensitive DNA-Biomacromolecule Sensor for the Detection Application of Clinical Cancer Samples.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Biosensors for Point Mutation Detection.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensitive and specific DNA hybridization is essential for nucleic acid chemistry. Competitive composition of probe and blocker has been the most adopted probe design for its relatively high sensitivity and specificity. However, the sensitivity and specificity were inversely correlated over the length and concentration of the blocker strand, making the optimization process cumbersome. Herein, we construct a theoretical model for competitive DNA hybridization, which disclose that both the thermodynamics and kinetics contribute to the inverse correlation. Guided by this, we invent the 4-way Strand Exchange LEd Competitive DNA Testing (SELECT) system, which breaks up the inverse correlation. Using SELECT, we identified 16 hot-pot mutations in human genome under uniform conditions, without optimization at all. The specificities were all above 140. As a demonstration of the clinical practicability, we develop probe systems that detect mutations in human genomic DNA extracted from ovarian cancer patients with a detection limit of 0.1%.
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