Evidence map›Paper›PMID 31611397›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2019

A mesophilic cysteine-less split intein for protein

Maniraj Bhagawati, Tobias M E Terhorst, Friederike Füsser, Simon Hoffmann, Tim Pasch, Shmuel Pietrokovski, Henning D Mootz

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Int&in: A machine learning-based web server for active split site identification in inteins.Protein science : a publication of the Protein Society · 2024
    Article
  8. Article
  9. Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization.Molecular therapy : the journal of the American Society of Gene Therapy · 2023
    Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. A Chemical Biology Primer for NMR Spectroscopists.Journal of magnetic resonance open · 2022
    Article
  15. ACS chemical biology · 2022
    Article
  16. Inteins as Drug Targets and Therapeutic Tools.Frontiers in molecular biosciences · 2022
    Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maniraj BhagawatiInstitute of Biochemistry, University of Münster, 48149 Münster, Germany.ORCID 0000-0002-3975-2264
Tobias M E TerhorstInstitute of Biochemistry, University of Münster, 48149 Münster, Germany.
Friederike FüsserInstitute of Biochemistry, University of Münster, 48149 Münster, Germany.
Simon HoffmannInstitute of Biochemistry, University of Münster, 48149 Münster, Germany.
Tim PaschInstitute of Biochemistry, University of Münster, 48149 Münster, Germany.ORCID 0000-0002-8528-983X
Shmuel PietrokovskiDepartment of Molecular Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel shmuel.pietrokovski@weizmann.ac.il Henning.Mootz@uni-muenster.de.
Henning D MootzInstitute of Biochemistry, University of Münster, 48149 Münster, Germany; shmuel.pietrokovski@weizmann.ac.il Henning.Mootz@uni-muenster.de.ORCID 0000-0002-3385-0234

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Split intein-mediated protein

Indexed as

Protein SplicingAmino Acid SequenceCysteineGenetic EngineeringHeLa CellsHumansInteinsOxidation-ReductionPeptide FragmentsTemperatureCysteinePeptide Fragmentschemical biologyfluorescent labelingprotein ligationthiol bioconjugation

Identifiers

PMID31611397
PMCPMC6825276

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.