ArticleProceedings of the National Academy of Sciences of the United States of America2019
AFF1 acetylation by p300 temporally inhibits transcription during genotoxic stress response.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Stabilization of AFF1 by PARylation ensures transcriptional restart after DNA damage.Nature chemical biology · 2026Article
- Dynamic partitioning of a critical elongation factor between LEC and SEC regulates cellular snRNA and proliferation-related mRNA transcription.Nature communications · 2026Article
- Post-translational modification-dependent oligomerization switch in regulation of global transcription and DNA damage repair during genotoxic stress.Nature communications · 2024Article
- Degradation of CDK9 by Ubiquitin E3 Ligase STUB1 Regulates P-TEFb Level and Its Functions for Global Target Gene Expression within Mammalian Cells.Molecular and cellular biology · 2023Article
- ATM-mediated ELL phosphorylation enhances its self-association through increased EAF1 interaction and inhibits global transcription during genotoxic stress.Nucleic acids research · 2022Article
- Negative Feedback Loop Mechanism between EAF1/2 and DBC1 in Regulating ELL Stability and Functions.Molecular and cellular biology · 2022Article
- Human FKBP5 Negatively Regulates Transcription through Inhibition of P-TEFb Complex Formation.Molecular and cellular biology · 2022Article
- Acetylation-dependent regulation of BRAF oncogenic function.Cell reports · 2022Article
- The CBP-1/p300 Lysine Acetyltransferase Regulates the Heat Shock Response inFrontiers in aging · 2022Article
- Circular RNA in Acute Central Nervous System Injuries: A New Target for Therapeutic Intervention.Frontiers in molecular neuroscience · 2022Review
- Genetic and Molecular Characterization Revealed the Prognosis Efficiency of Histone Acetylation in Pan-Digestive Cancers.Journal of oncology · 2022Article
- Differential microRNA Expression inJournal of clinical medicine · 2021Article
- DBC1, p300, HDAC3, and Siah1 coordinately regulate ELL stability and function for expression of its target genes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells.The Journal of biological chemistryArticle
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Soon after exposure to genotoxic reagents, mammalian cells inhibit transcription to prevent collisions with repair machinery and to mount a proper DNA damage response. However, mechanisms underlying early transcriptional inhibition are poorly understood. In this report, we show that site-specific acetylation of super elongation complex (SEC) subunit AFF1 by p300 reduces its interaction with other SEC components and impairs P-TEFb-mediated C-terminal domain phosphorylation of RNA polymerase II both in vitro and in vivo. Reexpression of wild-type AFF1, but not an acetylation mimic mutant, restores SEC component recruitment and target gene expression in AFF1 knockdown cells. Physiologically, we show that, upon genotoxic exposure, p300-mediated AFF1 acetylation is dynamic and strongly correlated with concomitant global down-regulation of transcription-and that this can be reversed by overexpression of an acetylation-defective AFF1 mutant. Therefore, we describe a mechanism of dynamic transcriptional regulation involving p300-mediated acetylation of a key elongation factor during genotoxic stress.
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