Use of a fluorescence assay to determine relative affinities of semisynthetic aminoglycosides to small RNAs representing bacterial and mitochondrial A sites.

Prabuddha Waduge, Girish C Sati, David Crich, Christine S Chow

Open access · greenAbstract read

In one paragraph

Article in Bioorganic & medicinal chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.3field-weighted citation impact, top 40% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

Small molecule-RNA targeting: starting with the fundamentals.Chemical communications (Cambridge, England) · 2020
Article
Frameworks for targeting RNA with small molecules.The Journal of biological chemistry
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Prabuddha WadugeDepartment of Chemistry, Wayne State University, Detroit, MI 48202, USA.

Girish C SatiDepartment of Chemistry, Wayne State University, Detroit, MI 48202, USA.

David CrichDepartment of Chemistry, Wayne State University, Detroit, MI 48202, USA.

Christine S ChowDepartment of Chemistry, Wayne State University, Detroit, MI 48202, USA. Electronic address: cchow@wayne.edu.

Wayne State University · US

Funding

Shaping Next Generation Aminoglycoside Antibiotics for Treatment of Multidrug-Resistant DiseasesR01AI123352 · NIAID · WAYNE STATE UNIVERSITY · PI CRICH, DAVID · 2016 to 2019

$2.7M

NIAID NIH HHS R01 AI123352

6 · The paper itself

Abstract

The off-target binding of aminoglycosides (AGs) to the A site of human mitochondrial ribosomes in addition to bacterial ribosomes causes ototoxicity and limits their potential as antibiotics. A fluorescence assay was employed to determine relative binding affinities of classical and improved AG compounds to synthetic RNA constructs representing the bacterial and mitochondrial A sites. Results compared well with previously reported in vitro translation assays with engineered ribosomes. Therefore, the minimal RNA motifs and fluorescence assay are shown here to be useful for assessing the selectivity of new compounds.

Indexed as

AminoglycosidesBacteriaBinding SitesFluorescenceHumansMitochondriaRNAAminoglycosidesRNAAminoglycosidesFluorescenceHelix 44Mitochondrial A siteOtotoxicity

Identifiers

PMID31610941

DOI10.1016/j.bmc.2019.115121

PMCPMC6961810

OpenAlexW2972808239

What OpenQuestion holds

Textmetadata

LicenceTDM

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.