ArticleJournal of neuroscience methods2020
Monitoring translation in synaptic fractions using a ribosome profiling strategy.
Article in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The trial behind it
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- mRNA and circRNA mislocalization to synapses are key features of Alzheimer's disease.PLoS genetics · 2024Article
- Utilities of Isolated Nerve Terminals in Ex Vivo Analyses of Protein Translation in (Patho)physiological Brain States: Focus on Alzheimer's Disease.Molecular neurobiology · 2024Review
- RNA in axons, dendrites, synapses and beyond.Frontiers in molecular neuroscience · 2024Review
- Control of Selective mRNA Translation in Neuronal Subcellular Compartments in Health and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Review
- Mnk1/2 kinases regulate memory and autism-related behaviours via Syngap1.Brain : a journal of neurology · 2023Article
- Reversal of memory and autism-related phenotypes inFrontiers in cell and developmental biology · 2023Article
- The interplay between translational efficiency, poly(A) tails, microRNAs, and neuronal activation.RNA (New York, N.Y.) · 2022Article
- Local Protein Translation and RNA Processing of Synaptic Proteins in Autism Spectrum Disorder.International journal of molecular sciences · 2021Review
- Functional Genomics of Axons and Synapses to Understand Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe aim of this study was to develop a method to study genome-wide local translation in biochemically isolated synaptic fractions (synaptoneurosomes). This methodology is of particular interest for neurons, due to the cardinal role of local translational control in neuronal sub-compartments, such as dendrites, for plasticity, learning, memory, and for disorders of the nervous system. NEW
methodWe combined established methods for purifying synaptoneurosomes with translational profiling (ribosome profiling), a method that employs unbiased next generation sequencing to simultaneously assess transcription and translation in a single sample.
resultsThe two existing methods are compatible to use in combination and yield high quality sequencing data, which are specific to synaptic compartments. This new protocol provides an easy to implement workflow, which combines biochemical isolation of synaptoneurosomes of varying levels of purity (crude or Percoll gradient purified) with the use of a commercial kit to generate sequencing libraries. COMPARISON WITH EXISTING
methodsCompared to previous studies of the synaptic translatome, our method shows less contamination with non-neuronal cell types or non-synaptic compartments, increasing the specificity of the data obtained.
conclusionsCombining the isolation of functional synaptic units with ribosome profiling offers a powerful tool to study local translation in synaptic compartments both in health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.