ArticleFrontiers in physiology2019
Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys.
Article in Frontiers in physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Harnessing the power of miRNAs for precision diagnosis and treatment of male infertility.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Testis tissue cryopreservation may be considered in boys with cryptorchidism.Asian journal of andrology · 2024Article
- Male Infertility: New Developments, Current Challenges, and Future Directions.The world journal of men's health · 2024Review
- Optimized Recovery of Immature Germ Cells after Prepubertal Testicular Tissue Digestion and Multi-Step Differential Plating: A Step towards Fertility Restoration with Cancer-Cell-Contaminated Tissue.International journal of molecular sciences · 2023Article
- Favorable culture conditions for spermatogonial propagation in human and non-human primate primary testicular cell cultures: a systematic review and meta-analysis.Frontiers in cell and developmental biology · 2023Article
- Clinical aspects of histological and hormonal parameters in boys with cryptorchidism: Thesis for PhD degree.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Article
- Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches.Frontiers in endocrinology · 2022Review
- Sertoli and Germ Cells Within Atrophic Seminiferous Tubules of Men With Non-Obstructive Azoospermia.Frontiers in endocrinology · 2022Article
- Article
- Fertility preservation in boys: recent developments and new insightsHuman reproduction open · 2020Article
- Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGonadotoxic treatment of malignant diseases as well as some non-malignant conditions such as cryptorchidism in young boys may result in infertility and failure to father children later in life. As a fertility preserving strategy, several centers collect testicular biopsies to cryopreserve spermatogonial stem cells (SSCs) world-wide. One of the most promising therapeutic strategies is to transplant SSCs back into the seminiferous tubules to initiate endogenous spermatogenesis. However, to obtain sufficient numbers of SSC to warrant transplantation, MATERIALS AND
methodsA minute amount of testicular biopsies (between 5 mg and 10 mg) were processed by mechanical and enzymatic digestion. SSCs were enriched by differential plating method in StemPro-34 medium supplemented with several growth factors. SSC-like cell clusters (SSCLCs) were passaged five times. SSCLCs were identified by immunohistochemical and immunofluorescence staining, using protein expression patterns in testis biopsies as reference. Quantitative polymerase chain reaction analysis of SSC markers LIN-28 homolog A (LIN28A), G antigen 1 (GAGE1), promyelocytic leukemia zinc finger protein (PLZF), integrin alpha 6 (ITGA6), ubiquitin carboxy-terminal hydrolase L1 (UCHL1) and integrin beta 1 (ITGB1) were also used to validate the SSC-like cell identity.
resultsProliferation of SSCLCs was achieved. The presence of SSCs in SSCLCs was confirmed by positive immunostaining of LIN28, UCHL1 and quantitative polymerase chain reaction for LIN28A, UCHL1, PLZF, ITGA6, and ITGB1, respectively.
conclusionThis study has demonstrated that SSCs from infant boys possess the capacity for
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