ArticleReproductive medicine and biology2019
In vitro spermatogenesis in two-dimensionally spread mouse testis tissues.
Article in Reproductive medicine and biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- An adverse outcome pathway-aligned ex vivo mouse testicular organ culture platform for mechanistic integration of multi-level endpoints with recovery assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.PloS one · 2026Article
- A long-term mouse testis organ culture system to identify germ cell damage induced by chemotherapy.Current research in toxicology · 2025Article
- Application of testicular organ culture system for the evaluation of spermatogenesis impairment.Scientific reports · 2024Article
- Alkylating agents-induced gonadotoxicity in prepubertal males: Insights on the clinical and preclinical front.Clinical and translational science · 2024Review
- Development of a novel testis-on-a-chip that demonstrates reciprocal crosstalk between Sertoli and Leydig cells in testicular tissue.Experimental & molecular medicine · 2024Article
- Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control.The Journal of reproduction and development · 2024Article
- Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.Scientific reports · 2023Article
- Generation of rat offspring using spermatids produced through in vitro spermatogenesis.Scientific reports · 2023Article
- Article
- Complete meiosis in rat prepubertal testicular tissue under in vitro sequential culture conditions.Andrology · 2023Article
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Androgen-regulatediScience · 2022Article
- Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies†.Biology of reproduction · 2022Review
- Biomaterial strategies for the application of reproductive tissue engineering.Bioactive materials · 2022Review
- Mesenchymal stem cells promote spermatogonial stem/progenitor cell pool and spermatogenesis in neonatal mice in vitro.Scientific reports · 2022Article
- A modified hydrostatic microfluidic pumpless device for in vitro murine ovarian tissue culture as research model for fertility preservation.Obstetrics & gynecology science · 2022Article
- Mini Bioreactor Can SupportCell journal · 2022Article
- Microfluidic and Static Organotypic Culture Systems to SupportFrontiers in physiology · 2022Article
- Influence of hydrogel encapsulation during cryopreservation of ovarian tissues and impact of post-thawing in vitro culture systems in a research animal model.Clinical and experimental reproductive medicine · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air. In this condition, however, a tissue piece tends to round up to be compact, whose central region suffers from shortage of nutrients and oxygen. In this study, the authors improved the culture condition by spreading each tissue thin and flat, by which they were able to get better access to the oxygen and nutrients.
methodsImmature mouse testis tissues placed on agarose gel block were forced to spread flat by covering with a polydimethylsiloxane (PDMS) ceiling chip (PC chip). They were then cultured for weeks and evaluated by the transgene expression of
resultsTestis tissues covered with PC chip initiated and maintained spermatogenesis in its wider region than those without PC chip covering. Flow cytometric analysis demonstrated that the PC method yielded more numerous meiotic germ cells than those without PC. Immunohistochemical examination confirmed the authentic histological figure of spermatogenesis from spermatogonia up to round or elongating spermatids.
conclusionsThe PC chip method is simple and effective to improve the efficiency of in vitro spermatogenesis in the organ culture system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.