ArticleCell journal2020
The Effect of Melatonin on Mitochondrial Function and Autophagy in In Vitro Matured Oocytes of Aged Mice.
Article in Cell journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Nutritional Regulation of Ovarian Bioenergetics: Implications for Reproductive Aging and Female Infertility.Nutrients · 2026Review
- Melatonin During Pre-Maturation and Its Effects on Bovine Oocyte Competence.Antioxidants (Basel, Switzerland) · 2025Article
- Hallmarks of female reproductive aging in physiologic aging mice.Nature aging · 2024Review
- Effect of melatonin on developmental competence, mitochondrial distribution, and intensity of fresh and vitrified/thawed in vitro matured buffalo oocytes.Reproductive biology and endocrinology : RB&E · 2024Article
- Article
- Insight of Melatonin: The Potential of Melatonin to Treat Bacteria-Induced Mastitis.Antioxidants (Basel, Switzerland) · 2022Review
- Mitochondria: emerging therapeutic strategies for oocyte rescue.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- In vitro maturation medium supplementation: utilization of repaglinide, L-carnitine, and mesenchymal stem cell-conditioned medium to improve developmental competence of oocytes derived from endometriosis mouse models.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2022Article
- The Role of Mitochondria in Oocyte Maturation.Cells · 2021Review
- Melatonin: Multi-Target Mechanism Against Diminished Ovarian Reserve Based on Network Pharmacology.Frontiers in endocrinology · 2021Article
- Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes culturedVeterinary world · 2021Article
- IGF2 improves the developmental competency and meiotic structure of oocytes from aged mice.Aging · 2020Article
- Quercetin promotes in vitro maturation of oocytes from humans and aged mice.Cell death & disease · 2020Article
- ASB7 Is a Novel Regulator of Cytoskeletal Organization During Oocyte Maturation.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study examined the MATERIALS AND
methodsIn this experimental study, germinal vesicles (GV) oocytes were collected from aged (with the age of six-months-old) and young mice (with age range of 6-8 weeks old) and then cultured in the in vitro culture medium (IVM) for 24 hours to each metaphase II (MII) oocytes and then supplemented with melatonin at a concentration of 10 μM. The culture medium of MII oocytes was devoid of melatonin. Afterward, the expression of the SIRT-1 and LC3 was assessed by immunocytochemistry. ATP-dependent luciferin-luciferase bioluminescence assay was employed for the measurement of the ATP contents. Intracellular reactive oxygen specious (ROS) was detected by DCFH-DA, and the total antioxidant capacity (TAC) level was determined by TAC assay.
resultsThe expression of SIRT-1 and LC3, as well as the measurement of the ATP content, was significantly increased in oocytes treated with melatonin compared with the oocytes receiving no treatment. Moreover, TAC was considerably higher in melatonin-treated oocytes than oocytes receiving no treatment. On the other hand, the level of ROS was significantly decreased in oocytes treated with melatonin in comparison with the untreated oocytes. The results indicated that melatonin considerably improved the development of oocytes as well.
conclusionAccording to the data, melatonin increased mitochondrial function and autophagy via an increase in the expression of SIRT1 and LC3, as well as the ATP contents while it decreased the levels of ROS and increased TAC in oocytes derived from aged mice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.