Evidence map›Paper›PMID 31606078›Full record

ArticleMethods in enzymology2019

Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists.

Sourav Chatterjee, Tesia N Stephenson, Austen L Michalak, Kamil Godula, Mia L Huang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Glycosaminoglycans.Advances in experimental medicine and biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Sourav ChatterjeeDepartment of Molecular Medicine, Scripps Research, Jupiter, FL, United States.
Tesia N StephensonDepartment of Molecular Medicine, Scripps Research, Jupiter, FL, United States.
Austen L MichalakDepartment of Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA, United States.
Kamil GodulaDepartment of Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA, United States. Electronic address: kgodula@ucsd.edu.
Mia L HuangDepartment of Molecular Medicine, Scripps Research, Jupiter, FL, United States; Department of Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA, United States. Electronic address: miahuang@scripps.edu.
Scripps Research Institute · USUniversity of California, San Diego · US

Funding

Bridging the Glycome and Proteome with Chemical BiologyR35GM142462 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI Mia L Huang · 2021 to 2026
$2.9M
In vivo glycan engineering at the cell-matrix interface to control stem cell fateDP2HD087954 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GODULA, KAMIL · 2015 to 2015
$2.3M
Chemical editing of nanoscale proteoglycan architecture and organization to control cellularR00HD090292 · NICHD · SCRIPPS FLORIDA · PI HUANG, MIA L · 2018 to 2020
$900k
Chemical editing of nanoscale proteoglycan architecture and organization to control cellular differentiationK99HD090292 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HUANG, MIA L · 2017 to 2017
$130k
NICHD NIH HHS DP2 HD087954NICHD NIH HHS K99 HD090292NICHD NIH HHS R00 HD090292NIGMS NIH HHS R35 GM142462
6 · The paper itself

Abstract

Glycosylation is a ubiquitous post-translational modification that decorates proteins and lipids with glycans. These glycans can play critical roles in regulating biological events, and therefore, the discovery of strategies that target these molecules represent an important advancement toward understanding and controlling glycan-mediated cellular phenotypes. We describe the use of a small molecule, surfen, to temporarily silence the functions mediated by heparan sulfate glycosaminoglycans in mouse embryonic stem cells. Surfen binds heparan sulfate to antagonize growth factor interactions, thereby inhibiting signal transduction events that lead to differentiation. The strategies outlined in this chapter allow the characterization of resulting antagonistic effects caused by glycan-small molecule binding events toward maintaining embryonic stem cell pluripotency, curbing differentiation, and inhibiting signaling events.

Indexed as

AnimalsCell Culture TechniquesCell DifferentiationCells, CulturedElectrophoresis, Polyacrylamide GelGlycosaminoglycansHeparan SulfateIntercellular Signaling Peptides and ProteinsMiceMouse Embryonic Stem CellsPolymerase Chain ReactionSignal TransductionUreaaminoquinurideGlycosaminoglycansHeparan SulfateIntercellular Signaling Peptides and ProteinsUreaGlycansGlycosaminoglycansGlycosylationSmall moleculeStem cells

Identifiers

PMID31606078
PMCPMC7265920
OpenAlexW2963958966

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.