ArticleMethods in enzymology2019
Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins.
Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Efficient splicing of the CPE intein derived from directed evolution of theActa biochimica et biophysica Sinica · 2023Article
- Harnessing Split-Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells.Chembiochem : a European journal of chemical biology · 2023Article
- Non-enzymatic covalent modifications: a new link between metabolism and epigenetics.Protein & cell · 2020Review
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Authors and funding
4 authors.
Funding
Abstract
Many cellular processes as well as their associated pathologies are regulated by protein post-translational modifications (PTMs). Understanding the precise roles of these adducts hinges on the development of methods to robustly and site-specifically manipulate proteins in their physiological environments. Recently, ultrafast intein protein trans-splicing (PTS) was harnessed to incorporate site-specific modifications on cellular chromatin in live cells. In this chapter, we present the protocols for the generation of synthetic modifications on native chromatin as well as highlight the capabilities of this methodology.
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