ArticleBMC genomics2019
Genomic characterization of Haemophilus influenzae: a focus on the capsule locus.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Pangenomic analysis reveals metabolic adaptation ofVirulence · 2026Article
- Epidemiology and antimicrobial resistance trends inMicrobiology spectrum · 2026Article
- Genetic population structure of Haemophilus influenzae at local and global scales.Nature microbiology · 2025Article
- A novel capsular operon and potentially conjugative plasmids in extensively drug-resistant urogenitalFrontiers in microbiology · 2025Article
- Trends in serotype distribution and antimicrobial susceptibility pattern of invasive Haemophilus influenzae isolates from Brazil, 2009-2021.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Clinical epidemiology and impact of Haemophilus influenzae airway infections in adults with cystic fibrosis.BMC infectious diseases · 2024Article
- Development and implementation of a core genome multilocus sequence typing scheme forMicrobial genomics · 2024Article
- Rare serotype c Haemophilus influenzae invasive isolate: characterization of the first case in Portugal.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications.Mikrochimica acta · 2023Article
- Comparative Genomic Analysis Reveals Genetic Diversity and Pathogenic Potential of Haemophilus seminalis and Emended Description of Haemophilus seminalis.Microbiology spectrum · 2023Article
- Epidemiology of Invasive Nontypeable Haemophilus influenzae Disease-United States, 2008-2019.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Secondary Cases of Invasive Disease Caused by Encapsulated and Nontypeable Haemophilus influenzae - 10 U.S. Jurisdictions, 2011-2018.MMWR. Morbidity and mortality weekly report · 2023Article
- National Danish surveillance of invasive clinicalFrontiers in microbiology · 2023Article
- Immunoinformatics design of multi-epitope peptide-based vaccine againstNetwork modeling and analysis in health informatics and bioinformatics · 2023Article
- Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies.Infection and drug resistance · 2023Review
- The natural history and genetic diversity of Haemophilus influenzae infecting the airways of adults with cystic fibrosis.Scientific reports · 2022Article
- Impact of a Single-Tube PCR Assay for the Detection ofMicroorganisms · 2022Article
- Antimicrobial Susceptibility Survey of Invasive Haemophilus influenzae in the United States in 2016.Microbiology spectrum · 2022Article
- Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.Journal of clinical microbiology · 2022Article
- Whole genome sequencing-based classification of human-related Haemophilus species and detection of antimicrobial resistance genes.Genome medicine · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHaemophilus influenzae (Hi) can cause invasive diseases such as meningitis, pneumonia, or sepsis. Typeable Hi includes six serotypes (a through f), each expressing a unique capsular polysaccharide. The capsule, encoded by the genes within the capsule locus, is a major virulence factor of typeable Hi. Non-typeable (NTHi) does not express capsule and is associated with invasive and non-invasive diseases.
methodsA total of 395 typeable and 293 NTHi isolates were characterized by whole genome sequencing (WGS). Phylogenetic analysis and multilocus sequence typing were used to characterize the overall genetic diversity. Pair-wise comparisons were used to evaluate the capsule loci. A WGS serotyping method was developed to predict the Hi serotype. WGS serotyping results were compared to slide agglutination (SAST) or real-time PCR (rt-PCR) serotyping.
resultsIsolates of each Hi serotype clustered into one or two subclades, with each subclade being associated with a distinct sequence type (ST). NTHi isolates were genetically diverse, with seven subclades and 125 STs being detected. Regions I and III of the capsule locus were conserved among the six serotypes (≥82% nucleotide identity). In contrast, genes in Region II were less conserved, with only six gene pairs from all serotypes showing ≥56% nucleotide identity. The WGS serotyping method was 99.9% concordant with SAST and 100% concordant with rt-PCR in determining the Hi serotype.
conclusionsGenomic analysis revealed a higher degree of genetic diversity among NTHi compared to typeable Hi. The WGS serotyping method accurately predicted the Hi capsule type and can serve as an alternative method for Hi serotyping.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.