ArticleMicrobial biotechnology2020
Droplet digital polymerase chain reaction for rapid broad-spectrum detection of bloodstream infections.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Testing for Bacteria and Fungi in Cells and Ancillary Reagents-Probe-Based Quantitative PCR Assays.Cell proliferation · 2026Article
- A fermented milk drink containingiScience · 2026Article
- A Nested PCR-Based Point of Care Testing of Multiplex Pathogens Associated with Bloodstream Infection.Pathogens (Basel, Switzerland) · 2026Article
- Multiplex droplet digital polymerase chain reaction for rapid diagnosing suspected bloodstream infections in patients with hematologic malignancies.Translational cancer research · 2026Article
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- Metagenomics next-generation sequencing of plasma combined with blood cells for improving the prognosis of early infection in patients with hematologic disorders: a real-world cohort study in northern China.Frontiers in molecular biosciences · 2026Article
- Digital Droplet PCR (ddPCR) for Absolute Quantification of 16S rRNA Copy Number in Metagenomic Data.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Surface bio-erosion and structural alteration of colored and agricultural plastics induced byFrontiers in microbiology · 2026Article
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- Joint application of multiplex drop-off digital PCR, droplet digital PCR, and metagenomic next-generation sequencing for the diagnosis of suspected infectious diseases: A retrospective cohort study.Journal of intensive medicine · 2025Article
- Optimizing Clinical Indicators in Hematologic Malignancies and Sepsis Using ddPCR: A Retrospective Study.Infectious diseases and therapy · 2025Article
- Performance of ddPCR-GNB for microbial diagnosis of suspected bloodstream infection due to the four most common gram-negative bacteria: a prospective, multicenter study.Microbiology spectrum · 2025Article
- Comparative analysis between digital PCR and blood culture for blood pathogen detection.Frontiers in medicine · 2025Article
- Comparison of microbial culture, metagenomic next-generation sequencing and droplet digital polymerase chain reaction methods for pathogen detection in patients with neurosurgical central nervous system infection.Frontiers in cellular and infection microbiology · 2025Article
- Plasma ddPCR for etiological diagnosis of focal bacterial infections.Frontiers in medicine · 2025Article
- Clinical evaluation of droplet digital pcr for suspected ascites infection in patients with liver cirrhosis.Hepatology international · 2024Article
- A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR.Heliyon · 2024Article
- Clinical evaluation of droplet digital PCR in the early identification of suspected sepsis patients in the emergency department: a prospective observational study.Frontiers in cellular and infection microbiology · 2024Observational
- Reliability of Droplet Digital PCR Alone and in Combination with Interleukin-6 and Procalcitonin for Prognosis of Bloodstream Infection.Infection and drug resistance · 2024Article
- Development and evaluation of a droplet digital PCR assay to detect Brucella in human whole blood.PLoS neglected tropical diseases · 2023Article
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8 authors.
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Abstract
The droplet digital polymerase chain reaction (ddPCR) is a novel molecular technique that allows rapid quantification of rare target DNA sequences. Aim of this study was to explore the feasibility of the ddPCR technique to detect pathogen DNA in whole blood and to assess the diagnostic accuracy of ddPCR to detect bloodstream infections (BSIs), benchmarked against blood cultures. Broad-range primers and probes were designed to detect bacterial 16S rRNA (and Gram stain for differentiation) and fungal 28S rRNA. To determine the detection limit of ddPCR, 10-fold serial dilutions of E. coli and C. albicans were spiked in both PBS and whole blood. The diagnostic accuracy of ddPCR was tested in historically collected frozen blood samples from adult patients suspected of a BSI and compared with blood cultures. Analyses were independently performed by two research analysts. Outcomes included sensitivity and specificity of ddPCR. Within 4 h, blood samples were drawn, and DNA was isolated and analysed. The ddPCR detection limit was approximately 1-2 bacteria or fungi per ddPCR reaction. In total, 45 blood samples were collected from patients, of which 15 (33%) presented with positive blood cultures. The overall sensitivity of ddPCR was 80% (95% CI 52-96) and specificity 87% (95% CI 69-96). In conclusion, the ddPCR technique has considerable potential and is able to detect very low amounts of pathogen DNA in whole blood within 4 h. Currently, ddPCR has a reasonable sensitivity and specificity, but requires further optimization to make it more useful for clinical practice.
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