ArticleScience (New York, N.Y.)2019
Ultrasound imaging of gene expression in mammalian cells.
Article in Science (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed, 251 citations in OpenAlex.
- Trimodal ultrasound imaging of brain-tumor interaction.iScience · 2026Article
- Endothelial Continuum and Capillary Specialization in Pulmonary Vascular Development.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Tailored Nanogel Network Topology Enables Clinical Ultrasound-Induced Mechanochemical Activation for In Vivo Therapy.Angewandte Chemie (International ed. in English) · 2026Article
- Monitoring in vivo transcription with synthetic serum markers.Nature communications · 2026Article
- Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.Nature communications · 2026Article
- Ultrasound-sensitive microrobotic sensor with robust anchoring for long-term digestive lesion assessment.National science review · 2026Article
- Gas vesicle-expressing human pluripotent stem cells enable multimodal ultrasound and optical coherence tomographic imaging.BMC biotechnology · 2026Article
- Deep excitation afterglow luminescent probes for biomedical applications.Chemical science · 2026Review
- Monitoring biological effects of somatic cell genome editing.Nature reviews. Genetics · 2026Review
- Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.Nature biotechnology · 2026Article
- Review
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Gas Vesicles and Acoustic Protein Nanostructures in Molecular Ultrasound Nanomedicine: Translational Archetypes, Biomaterial Design, and Barriers to Clinical Realization.International journal of nanomedicine · 2026Review
- Sonogenetics for precision medicine: from molecular toolkit to clinical translation.Theranostics · 2026Review
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability.Nature communications · 2025Article
- Multiplexed ultrasound imaging of gene expression.Nature methods · 2025Article
- Engineered acoustic reporters for simultaneous tracking of two variables on ultrasonography.Nature methods · 2025Article
- A New Radiotranscriptomic Approach to Analyze Combined Sets of T3b Stage-Specific Genes and Radiomic Features in Prostate Cancer.Cancer reports (Hoboken, N.J.) · 2025Article
- 3D Acoustic Imaging Hitting the Diffraction Limit via Fully Parameter-Optimized Meta-Lens and Frequency-Domain Reconstruction.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy.Synthetic and systems biotechnology · 2025Article
58 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in living animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.