ArticleAngewandte Chemie (International ed. in English)2019
Protein-Specific, Multicolor and 3D STED Imaging in Cells with DNA-Labeled Antibodies.
Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.Angewandte Chemie (International ed. in English) · 2026Article
- Quantitative Stimulated Emission Depletion (STED) Microscopy with DNA-Fluorophore Labels.ACS nano · 2026Article
- A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A bright and regenerative array tag with fluorogenic ligands for long-term live-cell nanoscopy and single-molecule tracking.Nature communications · 2025Article
- Rapid depletion and super-resolution microscopy reveal dual roles of SRSF5 in coordinating nuclear speckle-paraspeckle crosstalk during cellular stress.Nucleic acids research · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy.The journal of physical chemistry. B · 2024Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Neural network-assisted single-molecule localization microscopy with a weak-affinity protein tag.Biophysical reports · 2023Article
- Bleaching-resistant, Near-continuous Single-molecule Fluorescence and FRET Based on Fluorogenic and Transient DNA Binding.Chemphyschem : a European journal of chemical physics and physical chemistry · 2023Article
- Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.Journal of the American Chemical Society · 2023Article
- A new organic molecular probe as a powerful tool for fluorescence imaging and biological study of lipid droplets.Theranostics · 2023Article
- Article
- A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy.Nature communications · 2022Article
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging.Trends in biochemical sciences · 2021Review
- Transient Fluorescence Labeling: Low Affinity-High Benefits.International journal of molecular sciences · 2021Review
- Instant three-color multiplane fluorescence microscopy.Biophysical reports · 2021Review
- DNA hybridisation kinetics using single-molecule fluorescence imaging.Essays in biochemistry · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Photobleaching is a major challenge in fluorescence microscopy, in particular if high excitation light intensities are used. Signal-to-noise and spatial resolution may be compromised, which limits the amount of information that can be extracted from an image. Photobleaching can be bypassed by using exchangeable labels, which transiently bind to and dissociate from a target, thereby replenishing the destroyed labels with intact ones from a reservoir. Here, we demonstrate confocal and STED microscopy with short, fluorophore-labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to protein-specific antibodies. The constant exchange of fluorophore labels in DNA-based STED imaging bypasses photobleaching that occurs with covalent labels. We show that this concept is suitable for targeted, two-color STED imaging of whole cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.