ArticleMolecular plant pathology2019
Effector mining from the Erysiphe pisi haustorial transcriptome identifies novel candidates involved in pea powdery mildew pathogenesis.
Article in Molecular plant pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Structure-Based Comparative Analysis Reveals the Landscape of Powdery Mildew Secretomes Across Five Genera.Pathogens (Basel, Switzerland) · 2026Article
- Mechanism of Virulence in Fungal Pathogens with Diverse Modes of Living.Current microbiology · 2026Review
- Integrated transcriptomic and metabolomic analysis reveals the central role of phenylpropanoid biosynthesis in pea resistance to powdery mildew.Frontiers in plant science · 2026Article
- Sequence-based comparative secretome analysis reveals conserved core effectors and host lineage-specific divergence between monocot- and dicot-associated powdery mildew lineages.Frontiers in plant science · 2026Article
- Gaining insights into epigenetic memories through artificial intelligence and omics science in plants.Journal of integrative plant biology · 2025Review
- Functional Characterization ofFoods (Basel, Switzerland) · 2025Article
- Unraveling the Intricacies of Powdery Mildew: Insights into Colonization, Plant Defense Mechanisms, and Future Strategies.International journal of molecular sciences · 2025Review
- Genome and transcriptome analysis of Chinese wheat powdery mildew isolate 21-2 for discovery of important virulence determinants.Current research in microbial sciences · 2025Article
- A dual transcriptome analysis reveals accession-specific resistance responses inFrontiers in plant science · 2025Article
- Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.PLoS pathogens · 2024Article
- RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi.International journal of molecular sciences · 2023Review
- In Silico Characterization of the Secretome of the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Gene-Based Resistance toGenes · 2022Review
- Microbial interaction mediated programmed cell death in plants.3 Biotech · 2022Review
- The haustorium: The root of biotrophic fungal pathogens.Frontiers in plant science · 2022Review
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Gene Mining for Conserved, Non-Annotated Proteins ofJournal of fungi (Basel, Switzerland) · 2021Article
- Comparative analysis of extracellular proteomes reveals putative effectors of the boxwood blight pathogens, Calonectria henricotiae and C. pseudonaviculata.Bioscience reports · 2021Article
- The leucine-rich repeats in allelic barley MLA immune receptors define specificity towards sequence-unrelated powdery mildew avirulence effectors with a predicted common RNase-like fold.PLoS pathogens · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pea powdery mildew (PM) is an important fungal disease caused by an obligate biotroph, Erysiphe pisi (Ep), which significantly impacts pea production worldwide. The phytopathogen secretes a plethora of effectors, primarily through specialized infection structures termed haustoria, to establish a dynamic relationship with its host. To identify Ep effector candidates, a cDNA library of enriched haustoria from Ep-infected pea leaves was sequenced. The Ep transcriptome encodes 622 Ep candidate secreted proteins (CSPs), of which 167 were predicted to be candidate secreted effector proteins (CSEPs). Phylogenetic analysis indicates that Ep CSEPs are highly diverse, but, unlike cereal PM CSEPs, exhibit extensive sequence similarity with effectors from other PMs. Quantitative real-time PCR of a subset of EpCSEP/CSPs revealed that the majority are preferentially expressed in haustoria and exhibit infection stage-specific expression patterns. The functional roles of EpCSEP001, EpCSEP009 and EpCSP083 were probed by host-induced gene silencing (HIGS) via a double-stranded (ds) RNA-mediated RNAi approach. Foliar application of individual EpCSEP/CSP dsRNAs resulted in a marked reduction in PM disease symptoms. These findings were consistent with microscopic and molecular studies, suggesting that these Ep CSEP/CSPs play important roles in pea PM pathogenesis. Homology modelling revealed that EpCSEP001 and EpCSEP009 are analogous to fungal ribonucleases and belong to the RALPH family of effectors. This is the first study to identify and functionally validate candidate effectors from the agriculturally relevant pea PM, and highlights the utility of transcriptomics and HIGS to elucidate the key proteins associated with Ep pathogenesis.
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