ArticleExperimental and therapeutic medicine2019
Lovastatin increases the proliferation and osteoblastic differentiation of human gingiva-derived stem cells in three-dimensional cultures.
Article in Experimental and therapeutic medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 24 citations in OpenAlex.
- Laser-Engineered Multilayer Coatings Based on Zinc Oxide and Lovastatin-Functionalized Bioactive Glasses for Corrosion-Resistant and Antimicrobial Stainless Steel Implants.Biomimetics (Basel, Switzerland) · 2026Article
- [Research Progress on the Role and Regulatory Mechanisms of Lipid Metabolism in the Osteogenic/Odontogenic Differentiation of Dental Stem Cells].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Review
- Low-level laser therapy enhances osteogenic differentiation of gingiva-derived stem cells in 2D and 3D cultures.Scientific reports · 2025Article
- Lovastatin and Resveratrol Synergistically Improve Wound Healing and Inhibit Bacterial Growth.International journal of molecular sciences · 2025Article
- Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids.Medicina (Kaunas, Lithuania) · 2025Article
- Enhancement of Chondrogenic Differentiation in Bone Marrow-Derived Stem Cell Spheroids byJournal of personalized medicine · 2024Article
- Bone Morphogenetic Protein-9 Promotes Osteogenic Differentiation and Mineralization in Human Stem-Cell-Derived Spheroids.Medicina (Kaunas, Lithuania) · 2023Article
- Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells.Medicina (Kaunas, Lithuania) · 2023Article
- Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation.Medicina (Kaunas, Lithuania) · 2023Article
- In 2D and 3D Cell Culture Models, Effects of Endothelial Cells on E-cadherin / β-catenin Expression Levels and Spheroid Sizes in Ishikawa Cells.Asian Pacific journal of cancer prevention : APJCP · 2022Article
- Effects of noni on cellular viability and osteogenic differentiation of gingiva-derived stem cells demonstrated by RNA sequencing and quantitative PCR.Experimental and therapeutic medicine · 2022Article
- The Effects of Transforming Growth Factor-BioMed research international · 2022Article
- Article
- Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells.Medicina (Kaunas, Lithuania) · 2021Article
- Osteosphere Model to Evaluate Cell-Surface Interactions of Implantable Biomaterials.Materials (Basel, Switzerland) · 2021Article
- NELL-1 Increased the Osteogenic Differentiation and mRNA Expression of Spheroids Composed of Stem Cells.Medicina (Kaunas, Lithuania) · 2021Article
- Insulin-like growth factor 2-enhanced osteogenic differentiation of stem cell spheroids by regulation of Runx2 and Col1 expression.Experimental and therapeutic medicine · 2021Article
- Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review.Frontiers in immunology · 2021Review
- Effects of platelet-derived growth factor-BB on cellular morphology and cellular viability of stem cell spheroids composed of bone-marrow-derived stem cells.Biomedical reports · 2020Article
- Fibroblast growth factor-4 maintains cellular viability while enhancing osteogenic differentiation of stem cell spheroids in part by regulating RUNX2 and BGLAP expression.Experimental and therapeutic medicine · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lovastatin is a cholesterol-lowering agent that also has effects of cell proliferation and apoptosis. The present study was performed to evaluate the effects of lovastatin on the proliferation and osteogenic differentiation of three-dimensional cell spheroids formed from human gingiva-derived stem cells (GDSCs) using concave microwells. GDSCs were plated on polydimethylsiloxane-based concave micromolds and grown in the presence of lovastatin at concentrations of 0, 2 and 6 µM. The morphology of the cells was viewed under an inverted microscope, and cell viability was determined with Cell Counting kit-8 on days 2, 7 and 14. Alkaline phosphatase activity assays were performed to evaluate the osteogenic differentiation on days 2 and 8. Alizarin red-S staining was also used to assess the mineralization of the stem cell spheroids at day 14. The results confirmed that GDSCs formed spheroids in concave microwells. No significant changes were noted with longer incubation time, and no significant differences in cell viability were noted between the three lovastatin groups at each time point. Higher osteogenic differentiation was observed in the 2 µM group when compared with the control. Mineralized extracellular deposits were visible after Alizarin red-S staining, and higher mineralization was noted in the 2 and 6 µM lovastatin groups when compared with the 0 µM control. The relative mineralization values of the 0, 2 and 6 µM groups on day 14 were 39.0±9.6, 69.3±6.0 and 60.9±7.5, respectively. This study demonstrated that the application of lovastatin enhanced the osteogenic differentiation of cell spheroids formed from GDSCs. This suggests that combinations of lovastatin and stem cell spheroids may have the potential for use in tissue engineering.
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