Evidence map›Paper›PMID 31601965›Full record

ArticleScientific reports2019

Immobilisation of living coral embryos and larvae.

Carly J Randall, Christine Giuliano, David Mead, Andrew J Heyward, Andrew P Negri

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Carly J RandallAustralian Institute of Marine Science, PMB 3, Townsville, Queensland, 4810, Australia. c.randall@aims.gov.au.ORCID http://orcid.org/0000-0001-8112-3552
Christine GiulianoAustralian Institute of Marine Science, PMB 3, Townsville, Queensland, 4810, Australia.
David MeadAustralian Institute of Marine Science, PMB 3, Townsville, Queensland, 4810, Australia.
Andrew J HeywardAustralian Institute of Marine Science, Indian Ocean Marine Research Centre, University of Western Australia, 39 Fairway Street, Crawley, 6009, WA, Australia.ORCID http://orcid.org/0000-0002-8552-3665
Andrew P NegriAustralian Institute of Marine Science, PMB 3, Townsville, Queensland, 4810, Australia.ORCID http://orcid.org/0000-0003-1388-7395

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Embedding and immobilisation of living cells and microorganisms is used in a variety of research and commercial applications. Here we report the successful extended immobilisation of coral larvae in a low-gelling temperature agarose. Embryos and larvae of five broadcast-spawning Scleractinian species were immobilised in agarose gel and tested in a series of exploratory survival and settlement assays. The optimal developmental stage for immobilisation was after ciliation at approximately 24 hours post-fertilisation, after which, survival of immobilised larvae of all species was nearly 100%. In long-term assays, 50% of Montipora digitata larvae survived immobilised for 89 days. Furthermore, immobilised larvae of multiple species, that were released from the agarose, generally remained capable of settlement. These results demonstrate that the immobilisation of the early life-history stages of corals is possible for a variety of applications in basic and applied science.

Indexed as

AnimalsAnthozoaCoral ReefsDevelopmental BiologyFertilizationHydrogelsLarvaMicroscopy, FluorescenceTemperatureHydrogels

Identifiers

PMID31601965
PMCPMC6787016

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.