ArticleScientific reports2019
The interplay between chondrocyte spheroids and mesenchymal stem cells boosts cartilage regeneration within a 3D natural-based hydrogel.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Systematic Comparison of Biomaterials-Based Strategies for Osteochondral and Chondral Repair in Large Animal Models.Advanced healthcare materials · 2021Pooled it
- Evaluation of Cartilage Repair After Implantation of Labeled Human Chondrocytes as Free Cells or Spheroids in Rabbit Knees.International journal of molecular sciences · 2026Article
- Corrigendum to "Strong living scaffolds for load-bearing musculoskeletal tissue regeneration" [Mater. Today Bio 35 (2025) 102571].Materials today. Bio · 2026Article
- Co-Culture Approaches in Cartilage and Bone Tissue Regeneration.International journal of molecular sciences · 2025Review
- Use of Hydrogels in Regenerative Medicine: Focus on Mechanical Properties.International journal of molecular sciences · 2024Review
- Bioprinted High-Cell-Density Laminar Scaffolds Stimulate Extracellular Matrix Production in Osteochondral Co-Cultures.International journal of molecular sciences · 2024Article
- Rapidly Degrading Hydrogels to Support Biofabrication and 3D Bioprinting Using Cartilage Microtissues.ACS biomaterials science & engineering · 2024Article
- Enhanced chondrogenic potential in GelMA-based 3D cartilage model via Wnt3a surface immobilization.Scientific reports · 2024Article
- TiOGels (Basel, Switzerland) · 2024Article
- Regenerative mechanisms of stem cells and their clinical applications for degenerative eye diseases.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2024Review
- MegaPro, a clinically translatable nanoparticle forTheranostics · 2023Article
- An In Vitro Engineered Osteochondral Model as Tool to Study Osteoarthritis Environment.Advanced healthcare materials · 2023Article
- Changes of cell membrane fluidity for mesenchymal stem cell spheroids on biomaterial surfaces.World journal of stem cells · 2022Article
- Article
- Programming hydrogels to probe spatiotemporal cell biology.Cell stem cell · 2022Review
- Mechanically Competent Chitosan-Based Bioadhesive for Tendon-to-Bone Repair.Advanced healthcare materials · 2022Article
- Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink.Materials today. Bio · 2022Article
- Spheroid-Based Tissue Engineering Strategies for Regeneration of the Intervertebral Disc.International journal of molecular sciences · 2022Review
- Recent Biomimetic Approaches for Articular Cartilage Tissue Engineering and Their Clinical Applications: Narrative Review of the Literature.Advances in orthopedics · 2022Review
- The Emerging Use of ASC/Scaffold Composites for the Regeneration of Osteochondral Defects.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Articular cartilage (AC) lacks the ability to self-repair and cell-based approaches, primarily based on using chondrocytes and mesenchymal stem cells (MSCs), are emerging as effective technology to restore cartilage functionality, because cells synergic functionality may support the maintenance of chondrogenic phenotype and promote extracellular matrix regeneration. This work aims to develop a more physiologically representative co-culture system to investigate the influence of MSCs on the activity of chondrocytes. A thermo-sensitive chitosan-based hydrogel, ionically crosslinked with β-glycerophosphate, is optimised to obtain sol/gel transition at physiological conditions within 5 minutes, high porosity with pores diameter <30 µm, and in vitro mechanical integrity with compressive and equilibrium Young's moduli of 37 kPa and 17 kPa, respectively. Live/dead staining showed that after 1 and 3 days in culture, the encapsulated MSCs into the hydrogels are viable and characterised by round-like morphology. Furthermore chondrocyte spheroids, seeded on top of gels that contained either MSCs or no cells, show that the encapsulated MSCs stimulate chondrocyte activity within a gel co-culture, both in terms of maintaining the coherence of chondrocyte spheroids, leading to a larger quantity of CD44 (by immunofluorescence) and a higher production of collagen and glycosaminoglycans (by histology) compared with the mono-culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.