ArticleScientific reports2019
Stable reference genes for RT-qPCR analysis of gene expression in the Musa acuminata-Pseudocercospora musae interaction.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Selection of stable internal control genes for quantitative real-time PCR (RT-qPCR) in banana (Musa spp.) under short-term drought stress.Molecular biology reports · 2025Article
- Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data.Scientific reports · 2025Article
- Optimized SDS-Based Protocol for High-Quality RNA Extraction fromMethods and protocols · 2025Article
- Reference genes for RT-qPCR analysis in Musa acuminata genotypes contrasting in resistance to Fusarium oxysporum f. sp. cubense subtropical race 4.Scientific reports · 2024Article
- Evaluating the stability of host-reference gene expression and simultaneously quantifying parasite burden and host immune responses in murine malaria.Scientific reports · 2023Article
- Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications.Scientific reports · 2023Article
- Characterization of microRNAs and Target Genes inPlants (Basel, Switzerland) · 2023Article
- Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response.Frontiers in plant science · 2023Article
- Identification of RT-qPCR reference genes suitable for gene function studies in the pitaya canker disease pathogen Neoscytalidium dimidiatum.Scientific reports · 2022Article
- Transcriptome Profiling of the Resistance Response ofInternational journal of molecular sciences · 2022Article
- Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2022Article
- Evaluation of reference genes for transcript normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis inFrontiers in veterinary science · 2022Article
- A population based expression atlas provides insights into disease resistance and other physiological traits in cassava (Manihot esculenta Crantz).Scientific reports · 2021Article
- Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato 'Hawaii 7996' interactions.Scientific reports · 2021Article
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7 authors.
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Abstract
Leaf pathogens are limiting factors in banana (Musa spp.) production, with Pseudocercospora spp. responsible for the important Sigatoka disease complex. In order to investigate cellular processes and genes involved in host defence responses, quantitative real-time PCR (RT-qPCR) is an analytical technique for gene expression quantification. Reliable RT-qPCR data, however, requires that reference genes for normalization of mRNA levels in samples are validated under the conditions employed for expression analysis of target genes. We evaluated the stability of potential reference genes ACT1, α-TUB, UBQ1, UBQ2, GAPDH, EF1α, APT and RAN. Total RNA was extracted from leaf tissues of Musa acuminata genotypes Calcutta 4 (resistant) and Cavendish Grande Naine (susceptible), both subjected to P. musae infection. Expression stability was determined with NormFinder, BestKeeper, geNorm and RefFinder algorithms. UBQ2 and RAN were the most stable across all M. acuminata samples, whereas when considering inoculated and non-inoculated leaf samples, APT and UBQ2 were appropriate for normalization in Calcutta 4, with RAN and α-TUB most stable in Cavendish Grande Naine. This first study of reference genes for relative quantification of target gene expression in the M. acuminata-P. musae interaction will enable reliable analysis of gene expression in this pathosystem, benefiting elucidation of disease resistance mechanisms.
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